Ferencak Ivana, Gveric Grginic Ana, Juzbasic Tajana, Tabain Irena, Tonkic Marija, Goic-Barisic Ivana, Juric Dragan, Jankovic Hrvojka, Katic Luka, Novak Anita
Department of Microbiology, Croatian Institute of Public Health, 10000 Zagreb, Croatia.
Food-and Water-Borne Infections Study Group (EFWISG), European Society of Clinical Microbiology and Infectious Disease (ESCMID), 4051 Basel, Switzerland.
Pathogens. 2025 Apr 24;14(5):408. doi: 10.3390/pathogens14050408.
Mikawasima is a rare, mainly environmental serovar. In 2020, an outbreak was observed in neonatal and maternal wards of the University Hospital of Split and was established as an endemic until the end of 2024. Using whole-genome sequencing, this study aimed to analyse the phenotypic and genotypic characteristics of . Mikawasima isolates and to elaborate whether the spread of the same clone occurred. Sequenced isolates were classified as ST2030, with the presence of aminoglycoside and extended spectrum beta-lactam resistance genes. Ten percent of the sequenced isolates exhibit multi-drug resistance. Identified virulence factors that include biofilm formation genes suggest the potential persistence of . Mikawasima in the hospital environment, while spatial and temporal analysis reveal clonal expansion and possible horizontal transmission between different hospital wards. This study provides a deep understanding of the genomic composition of . Mikawasima and emphasises the need for more stringent infection prevention measures, especially in vulnerable neonatal and postpartum settings, to mitigate the risk of healthcare-associated infections, and it should be followed by further microbiological and epidemiological investigations to identify the source of infection.
御川岛是一种罕见的、主要与环境相关的血清型。2020年，在斯普利特大学医院的新生儿和产科病房观察到一次疫情爆发，并被确定为地方病，一直持续到2024年底。本研究利用全基因组测序分析御川岛分离株的表型和基因型特征，并阐述同一克隆株是否发生传播。测序的分离株被归类为ST2030，存在氨基糖苷类和超广谱β-内酰胺抗性基因。10%的测序分离株表现出多重耐药性。鉴定出的包括生物膜形成基因在内的毒力因子表明御川岛在医院环境中可能持续存在，而空间和时间分析揭示了克隆扩张以及不同医院病房之间可能的水平传播。本研究深入了解了御川岛的基因组组成，并强调需要采取更严格的感染预防措施，特别是在脆弱的新生儿和产后环境中，以降低医疗相关感染的风险，随后应进行进一步的微生物学和流行病学调查以确定感染源。
