长链非编码 RNA HOTTIP 通过抑制 miR-4301 促进鼻咽癌细胞的增殖、迁移和侵袭。
Long Noncoding RNA HOTTIP Promotes Nasopharyngeal Cancer Cell Proliferation, Migration, and Invasion by Inhibiting miR-4301.
机构信息
Department of Otolaryngology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China (mainland).
出版信息
Med Sci Monit. 2019 Jan 27;25:778-785. doi: 10.12659/MSM.912728.
BACKGROUND The long noncoding RNA (lncRNA) HOTTIP is involved in gastric cancer tumorigenesis, papillary thyroid carcinoma, colorectal cancer, lung adenocarcinoma, and hepatocellular carcinoma, but it is unclear how HOTTIP exerts roles in nasopharyngeal carcinoma (NPC). The present study investigated HOTTIP function during NPC development. MATERIAL AND METHODS HOTTIP levels in cancer specimens and cell lines were analyzed using qRT-PCR. HOTTIP function in NPC was determined by Cell Counting Kit-8 (CCK8) and Transwell assay. RESULTS HOTTIP expression was increased in NPC tissues. Higher levels of HOTTIP are correlated with lower survival in NPC patients. HOTTIP silencing suppressed the proliferation, cell cycle, migration, and invasion of NPC cells. HOTTIP served as a sponge for miR-4301. miR-4301 expression was significantly inhibited by HOTTIP in NPC cells. miR-4301 overexpression dramatically inhibited NPC cell proliferation, migration, and invasion. CONCLUSIONS This study showed that HOTTIP acts as an oncogene in NPC by sponging miR-4301.
背景
长链非编码 RNA（lncRNA）HOTTIP 参与胃癌肿瘤发生、甲状腺乳头状癌、结直肠癌、肺腺癌和肝细胞癌，但尚不清楚 HOTTIP 在鼻咽癌（NPC）中如何发挥作用。本研究探讨了 HOTTIP 在 NPC 发展过程中的作用。
方法
使用 qRT-PCR 分析癌症标本和细胞系中的 HOTTIP 水平。通过细胞计数试剂盒-8（CCK8）和 Transwell 测定法确定 NPC 中 HOTTIP 的功能。
结果
HOTTIP 在 NPC 组织中表达增加。HOTTIP 水平较高与 NPC 患者的生存率降低相关。HOTTIP 沉默抑制 NPC 细胞的增殖、细胞周期、迁移和侵袭。HOTTIP 可作为 miR-4301 的海绵。NPC 细胞中 HOTTIP 显著抑制 miR-4301 的表达。miR-4301 的过表达可显著抑制 NPC 细胞的增殖、迁移和侵袭。
结论
本研究表明，HOTTIP 通过海绵吸附 miR-4301 在 NPC 中发挥致癌基因作用。
Zotero 插件