Overexpression of lincRNA02471 promote cancer development though miR-758/HIPK3 signaling pathway in papillary thyroid cancer.

AIMS

In previous studies, numerous differential lncRNAs were identified via RNA-sequencing. In this dysregulated lncRNAs, lincRNA02471 attracted our attention due to its highest fold change. The aims of our study mainly focused on the function and mechanism of lincRNA02471 in papillary thyroid cancer.

MATERIALS AND METHODS

Overexpression and knockdown vectors were constructed to investigate the function of lincRNA02471. Proliferation, apoptosis, invasion and EMT were performed to assess the function of lincRNA02471. Dual-luciferase reporter assay was performed to explore the relationship between lincRNA02471 and miR-758.

RESULTS

We found that lincRNA02471 was manifestly upregulated in papillary cancer tissues. Overexpression of lincRNA02471 significantly promoted the cell proliferation, invasion and inhibited cell apoptosis. Knockdown of lincRNA02471 inhibited the cancer development. We also found that lincRNA02471 negatively regulate miR-758 in papillary thyroid cancer. miR-758 can restore the effect of lincRNA02471. Besides, we identified that HIPK3 was the direct target of miR-758.

CONCLUSION

we performed comprehensive study of lincRNA02471 and explore its function and mechanism in papillary thyroid cancer. lincRNA02471 can sponge miR-758 and positively regulate HIPK3 to promote papillary thyroid cancer development. Our study provides new target for clinical treatment and new clues for understanding the molecular mechanism of cancer development.