Rondon Silvia I, Roster Mary Sue, Hamlin Launa L, Green Kelsie J, Karasev Alexander V, Crosslin J M
Oregon State University, Hermiston Agricultural Research and Extension Center, Hermiston.
United States Department of Agriculture-Agricultural Research Service, Prosser, WA.
Plant Dis. 2016 Aug;100(8):1586-1590. doi: 10.1094/PDIS-10-15-1189-RE. Epub 2016 May 26.
The beet leafhopper, Circulifer tenellus, is an agriculturally important pest, particularly in the western United States. This insect transmits the Beet curly top virus (BCTV) to multiple crops, including bean, tomato, and pepper. In this study, we investigated the incidence of BCTV in individual leafhoppers collected at several sites in northeastern Oregon during the growing season in 2007, 2008, and 2009. Of the 800 insects tested, 151 (18.9%) were found positive for the virus. Percentage of virus incidence varied from 0% at one location in 2009 to a high of 55.6% for a location sampled in 2008. The complete virus genomes from one virus-positive insect collected in each of the 3 years were determined. BLAST analysis of the BCTV whole-genome sequences from 2007, 2008, and 2009 insects showed 98, 94, and 96% identities with the BCTV-Worland sequence (AY134867), respectively. The BCTV_2008 sequence showed the greatest identity (96%) with another BCTV genomic sequence (JN817383), and was found to be a recombinant between the BCTV-Worland type, representing the majority of the genome (approximately 2.2 kb), and the BCTV-CFH type that provided an approximately 0.8-kb fragment spanning replication-related genes C1 and C2. This area of the BCTV genome, between the C1 and C2 genes, was previously found to carry symptom determinants of the virus, and the data may suggest more severe effects of BCTV during the 2008 season. Results indicate that BCTV is common and widespread in C. tenellus in eastern Oregon and that there is substantial genetic diversity among the virus strains present in this important field and vegetable crop-growing region.
甜菜叶蝉（Circulifer tenellus）是一种对农业具有重要影响的害虫，在美国西部尤为如此。这种昆虫会将甜菜曲顶病毒（BCTV）传播给多种作物，包括豆类、番茄和辣椒。在本研究中，我们调查了2007年、2008年和2009年生长季节在俄勒冈州东北部多个地点采集的单个叶蝉中BCTV的感染率。在检测的800只昆虫中，有151只（18.9%）被检测出病毒呈阳性。病毒感染率从2009年一个地点的0%到2008年一个采样地点的高达55.6%不等。测定了从这3年中每年采集的一只病毒阳性昆虫的完整病毒基因组。对2007年、2008年和2009年昆虫的BCTV全基因组序列进行的BLAST分析显示，它们与BCTV-Worland序列（AY134867）的同源性分别为98%、94%和96%。BCTV_2008序列与另一个BCTV基因组序列（JN817383）的同源性最高（96%），并且被发现是BCTV-Worland型（代表基因组的大部分，约2.2 kb）和BCTV-CFH型之间的重组体，BCTV-CFH型提供了一个约0.8 kb的片段，跨越与复制相关的基因C1和C2。此前发现BCTV基因组中C1和C2基因之间的这个区域携带病毒的症状决定因素，这些数据可能表明2008年季节BCTV的影响更为严重。结果表明，BCTV在俄勒冈州东部的甜菜叶蝉中普遍存在且分布广泛，并且在这个重要的大田和蔬菜作物种植区中存在的病毒株之间存在大量的遗传多样性。
