Soler-Garzón Alvaro, Goldoff Deidrah, Thornton Alyson, Swisher Grimm Kylie D, Hart John P, Song Qijian, Strausbaugh Carl A, Miklas Phillip N
Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA, United States.
Global Pathology Support Platform, HM Clause Seed Company, Davis, CA, United States.
Front Plant Sci. 2023 Jul 14;14:1215950. doi: 10.3389/fpls.2023.1215950. eCollection 2023.
Beet curly top virus (BCTV), which is synonymous with curly top virus (CTV), causes significant yield loss in common bean (snap and dry beans) cultivars and several other important crops. Common bean cultivars have been found to be resistant to CTV, but screening for resistance is challenging due to the cyclical nature of epidemics and spotty feeding by the leafhopper that vectors the virus. We used an SNP dataset for the Snap Bean Association Panel (SnAP) agro-inoculated with CTV-Logan (CA/Logan) strain to locate the gene region to a 1.7-Mb interval on chromosome Pv07 using genome-wide association study (GWAS) analysis. Recombinant lines from the SnAP were used to further narrow the region to a 58.0-kb interval. A missense SNP (S07_2970381) in candidate gene Phvul.007G036300 Exonuclease V (EXO5) was identified as the most likely causal mutation, and it was the most significant SNP detected by GWAS in a dry bean population (DBP) naturally infected by the CTV-Worland (Wor) strain. Tm-shift assay markers developed for SNP S07_2970381 and two linked SNPs, S07_2970276 and S07_2966197, were useful for tracking different origins of the EXO5 candidate gene resistance to CTV in common bean. The three SNPs identified four haplotypes, with haplotype 3-1 (Haplo3-1) of Middle American origin associated with the highest levels of CTV resistance. This SNP-haplotype assay will enable breeders to track resistance sources and to develop cultivars with better CTV resistance.
甜菜曲顶病毒（BCTV），与曲顶病毒（CTV）同义，会导致菜豆（嫩荚豆和干豆）品种以及其他几种重要作物大幅减产。已发现菜豆品种对CTV具有抗性，但由于疫情的周期性以及传播该病毒的叶蝉的点状取食，抗性筛选颇具挑战性。我们使用了一个单核苷酸多态性（SNP）数据集，该数据集来自用CTV-洛根（加利福尼亚/洛根）株系进行农业接种的嫩荚豆关联面板（SnAP），通过全基因组关联研究（GWAS）分析将该基因区域定位到Pv07染色体上一个1.7兆碱基的区间。来自SnAP的重组系被用于进一步将该区域缩小到一个58.0千碱基的区间。候选基因Phvul.007G036300外切核酸酶V（EXO5）中的一个错义SNP（S07_2970381）被确定为最有可能的因果突变，并且它是在被CTV-沃兰（Wor）株系自然感染的干豆群体（DBP）中通过GWAS检测到的最显著的SNP。为SNP S07_2970381以及两个连锁SNP S07_2970276和S07_2966197开发的Tm-位移分析标记，有助于追踪菜豆中EXO5候选基因对CTV抗性的不同来源。这三个SNP确定了四种单倍型，其中源自中美洲的单倍型3-1（Haplo3-1）与最高水平的CTV抗性相关。这种SNP-单倍型分析将使育种者能够追踪抗性来源，并培育出具有更好CTV抗性的品种。
