Laboratory of Toxicology and Food Chemistry, Faculty of Pharmacy , University of Valencia , Burjassot 46100 , Valencia , Spain.
J Nat Prod. 2019 Feb 22;82(2):403-406. doi: 10.1021/acs.jnatprod.8b00283. Epub 2019 Jan 28.
The aim of the present work was to study the occurrence of mycotoxins [aflatoxins (1-4), 3-acetyldeoxyniavlenol (5), 15-acetyldeoxynivalenol (6), nivalenol (7), HT-2 (8), T-2 (9), ochratoxin A (10), zearalenone (11), enniatin A (12), enniatin A1 (13), enniatin B (14), enniatin B1 (15), and beauvericin (16)] present in potable products derived from herbal teas. Analysis was carried out by liquid chromatography coupled to ion-trap tandem mass spectrometry (LC-MS/MS-IT) after a dispersive liquid-liquid microextraction procedure (DLLME) was conducted. The DLLME method was applied to 52 commercial samples of chamomile, chamomile with anise, chamomile with honey, linden, pennyroyal mint, thyme, valerian, and horsetail beverages. The results obtained showed that the following mycotoxins were detected in the samples: 2 (19.1 to 134.7 μg/L), 3 (below the limit of quantification), and 4 (2.2 to 13.5 μg/L). Also, 6 was detected in one sample at 112.5 μg/L, and 14 was detected only in two samples, although at very low concentration levels. Pennyroyal mint and thyme showed the highest concentration levels of mycotoxins. A risk assessment, however, showed negative results regarding the consumption of herbal tea beverages and the presence of mycotoxins.
本研究旨在检测药茶类饮品中真菌毒素（黄曲霉毒素（1-4）、3-乙酰脱氧雪腐镰刀菌烯醇（5）、15-乙酰脱氧雪腐镰刀菌烯醇（6）、雪腐镰刀菌烯醇（7）、HT-2 毒素（8）、T-2 毒素（9）、赭曲霉毒素 A（10）、玉米赤霉烯酮（11）、恩镰孢菌素 A（12）、恩镰孢菌素 A1（13）、恩镰孢菌素 B（14）、恩镰孢菌素 B1（15）和展青霉素（16））的存在情况。样品经分散液液微萃取（DLLME）处理后，采用液相色谱-离子阱串联质谱法（LC-MS/MS-IT）进行分析。DLLME 法用于 52 份商业样品的检测，包括洋甘菊茶、茴芹洋甘菊茶、蜂蜜洋甘菊茶、椴花茶、薄荷茶、百里香茶、缬草茶和木贼茶。结果表明，在所检测的样品中，以下真菌毒素被检出：2（19.1 至 134.7μg/L）、3（低于定量限）和 4（2.2 至 13.5μg/L）。此外，112.5μg/L 的样品中检测到 6，仅在 2 个样品中检测到 14，但其浓度非常低。薄荷茶和百里香茶的真菌毒素浓度最高。然而，基于风险评估，草药茶类饮品的消费和真菌毒素的存在并不会带来风险。
