Khusro Ameer, Aarti Chirom, Barbabosa-Pilego Alberto, Rojas Hernández Saúl
a Research Department of Plant Biology and Biotechnology , Loyola College , Chennai , India.
b Facultad de Medicina Veterinaria y Zootecnia , Universidad Autónoma del Estado de México , Estado de México , Mexico.
Prep Biochem Biotechnol. 2019;49(2):176-183. doi: 10.1080/10826068.2019.1566149. Epub 2019 Jan 27.
The present investigation was aimed to determine the anti-pathogenic, antibiofilm, and technological properties of fermented food associated Staphylococcus succinus strain AAS2. The anti-pathogenic attribute of cell-free neutralized supernatant (CFNS) of strain AAS2 was assessed against food-borne and enteric pathogens that revealed promising activity against Staphylococcus aureus and Enterobacter aerogenes with high arbitrary unit of 220.25 ± 3.3 and 170.2 ± 4.6 AU/mL, respectively. Furthermore, the antibiofilm and time-kill assay of CFNS of strain AAS2 depicted remarkable reduction in biofilm formation of indicator pathogens in a dose-dependent manner. Moreover, time-kill assay data revealed the drastic reduction in the viability (log cfu/mL) of S. aureus and E. aerogenes in the presence of varied minimum inhibitory concentration ranges of CFNS. The distinct technological properties of strain AAS2 were demonstrated using standard methodologies. Reported results estimated moderate level of exopolysaccharide (41.3 ± 0.6 mg/L) and lipase production (8.3 ± 0.3 mm), followed by remarkable autolytic (30.1 ± 1.2-43.1 ± 1.3%), catalase (13.82 ± 0.3 AU), and nitrate reductase (10.25 ± 0.3 mM nitrite/mg dry weight) activities under standard conditions. Most importantly, the strain cleared the specific in vitro safety assessment tests. The described anti-pathogenic and technological traits of strain AAS2 paved the way to utilize it in pharmaceutical as well as food processing industries as starter/adjunct culture.
本研究旨在确定与发酵食品相关的琥珀酸葡萄球菌菌株AAS2的抗病原性、抗生物膜特性和工艺特性。对菌株AAS2的无细胞中和上清液（CFNS）的抗病原性进行了评估，以检测其对食源性病原体和肠道病原体的作用，结果显示该上清液对金黄色葡萄球菌和产气肠杆菌具有良好的活性，其任意单位分别高达220.25±3.3和170.2±4.6 AU/mL。此外，菌株AAS2的CFNS的抗生物膜和时间-杀菌试验表明，指示病原体的生物膜形成呈剂量依赖性显著减少。而且，时间-杀菌试验数据显示，在不同最低抑菌浓度范围的CFNS存在下，金黄色葡萄球菌和产气肠杆菌的活力（log cfu/mL）大幅降低。使用标准方法证明了菌株AAS2具有独特的工艺特性。报告结果显示，在标准条件下，该菌株产生的胞外多糖水平适中（41.3±0.6 mg/L），脂肪酶产量为（8.3±0.3 mm），同时具有显著的自溶活性（30.1±1.2 - 43.1±1.3%）、过氧化氢酶活性（13.82±0.3 AU）和硝酸还原酶活性（10.25±0.3 mM亚硝酸盐/毫克干重）。最重要的是，该菌株通过了特定的体外安全性评估测试。菌株AAS2所描述的抗病原性和工艺特性为其在制药和食品加工业中作为发酵剂/辅助培养物的应用铺平了道路。
