Research Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai-34, Tamil Nadu, India.
Department of Industrial Biotechnology, Bharath University, Selaiyur, Chennai - 73, India.
Arch Oral Biol. 2018 May;89:99-106. doi: 10.1016/j.archoralbio.2018.02.014. Epub 2018 Feb 23.
To investigate the probiotic characteristics, anti-Candida activity, and antibiofilm attributes of Hentak derived Lactobacillus pentosus strain LAP1.
The probiotic properties of strain LAP1 were depicted by adapting standard protocols. The anti-Candida and antibiofilm properties of isolate were determined using agar well diffusion assay and ELISA reader test, respectively. The time-kill assay was performed using viable colony count assay. Further, the co-aggregation property of strain LAP1 was determined based on standard methodology.
Strain LAP1 exhibited not only tolerance to acidic pH but also showed resistivity (P ≤ 0.05) to simulated gastric juice exposure. Similarly, the strain was able to tolerate bile salt, showed hyperproteolytic activity, and also depicted susceptibility to most of the antibiotics tested. Auto-aggregation phenomenon (37.5-60%), hydrophobicity nature (42.85%), and survival potentiality of strain LAP1 under freeze-dried condition (9.0 ± 0.01 log CFU/ml) made the isolate a promising probiotic candidate. Cell-free neutralized supernatant (CFNS) of strain LAP1 exhibited potent antifungal activities against C. albicans, C. tropicalis, and C. krusei with arbitrary unit of 150 ± 4.34, 200 ± 5.21, and 130 ± 5.13 AU/ml, respectively and depicted remarkable reduction in the biofilm formation of respective Candida sp. in a concentration dependent manner. Moreover, time-kill assay data provided the growth inhibition of all Candida sp. in a time dependent manner. Additionally, strain LAP1 revealed significant co-aggregate percentage with C. albicans, C. tropicalis, and C. krusei.
L. pentosus strain LAP1 exhibited a good probiotic characteristics, potent anti-Candida activity, and significant antibiofilm property that could be undoubtedly recommended for its vast applications not only in food industries but also as biotherapeutic agent against Candida infections in pharmaceutical industries.
研究源自亨塔克乳杆菌 pentosus 菌株 LAP1 的益生菌特性、抗念珠菌活性和抗生物膜特性。
通过适应标准方案来描述菌株 LAP1 的益生菌特性。使用琼脂孔扩散试验和 ELISA 读取器试验分别确定分离株的抗念珠菌和抗生物膜特性。使用活菌计数试验进行杀菌试验。此外，根据标准方法确定菌株 LAP1 的共聚特性。
菌株 LAP1 不仅表现出对酸性 pH 的耐受性，而且对模拟胃液暴露也表现出抗性（P≤0.05）。同样，该菌株能够耐受胆汁盐，表现出超蛋白水解活性，并且对测试的大多数抗生素表现出敏感性。菌株 LAP1 的自聚集现象（37.5-60%）、疏水性（42.85%）和在冻干条件下的生存能力（9.0±0.01 log CFU/ml）使该分离株成为有前途的益生菌候选物。菌株 LAP1 的无细胞中和上清液（CFNS）对 C. albicans、C. tropicalis 和 C. krusei 表现出强烈的抗真菌活性，任意单位分别为 150±4.34、200±5.21 和 130±5.13 AU/ml，并以浓度依赖的方式显著减少了各自念珠菌属的生物膜形成。此外，杀菌试验数据以时间依赖的方式提供了对所有念珠菌属的生长抑制。此外，菌株 LAP1 与 C. albicans、C. tropicalis 和 C. krusei 显示出显著的共聚百分比。
乳杆菌 pentosus 菌株 LAP1 表现出良好的益生菌特性、强大的抗念珠菌活性和显著的抗生物膜特性，不仅可以在食品工业中广泛应用，还可以作为制药工业中对抗念珠菌感染的生物治疗剂。
