Department of Dermatology, Seoul National University Boramae Medical Center, Seoul, Korea.
Department of Plastic and Reconstructive Surgery, Seoul National University Boramae Medical Center, Seoul, Korea.
Exp Dermatol. 2019 Mar;28(3):300-307. doi: 10.1111/exd.13895.
There is an unmet need in novel therapeutics for atopic dermatitis (AD). We examined the effects of autologous adipose-derived stem cells (ADSCs) on AD-like skin lesions induced by the application of 2,4-dinitrochlorobenzene (DNCB) in NC/Nga mice. Autologous ADSCs and ADSC-conditioned medium (ADSC-CM) were injected intralesionally three times. Clinical severity and histopathologic findings were compared in sham naïve control, saline-treated, ADSC-treated, ADSC-CM-treated and 2.5% cortisone lotion-applied animals. The severity index, skin thickness, mast cell number, as well as expression levels of thymic stromal lymphopoietin, CD45, chemoattractant receptor-homologous molecule, chemokine ligand 9 and chemokine ligand 20 were significantly lower in mice treated with ADSC, ADSC-CM, or 2.5% cortisone lotion. Tissue levels of interferon-γ as well as serum levels of interleukin-33 and immunoglobulin E levels were also decreased in those groups. We conclude that autologous ADSCs improved DNCB-induced AD-like skin lesions in NC/Nga mice by reducing inflammation associated with Th2 immune response and interferon-γ.
特应性皮炎（AD）的新型治疗方法存在未满足的需求。我们研究了自体脂肪来源干细胞（ADSCs）对 2,4-二硝基氯苯（DNCB）诱导的 NC/Nga 小鼠 AD 样皮肤损伤的影响。自体 ADSC 和 ADSC 条件培养基（ADSC-CM）三次注射到病变内。比较了假 naive 对照、盐水处理、ADSC 处理、ADSC-CM 处理和 2.5%皮质醇乳膏处理动物的临床严重程度和组织病理学发现。ADSC、ADSC-CM 或 2.5%皮质醇乳膏处理的小鼠严重程度指数、皮肤厚度、肥大细胞数量以及胸腺基质淋巴细胞生成素、CD45、趋化因子受体同源分子、趋化因子配体 9 和趋化因子配体 20 的表达水平均显著降低。这些组中的组织干扰素-γ水平以及血清白细胞介素-33 和免疫球蛋白 E 水平也降低。我们得出结论，自体 ADSC 通过减少与 Th2 免疫反应和干扰素-γ相关的炎症，改善了 NC/Nga 小鼠的 DNCB 诱导的 AD 样皮肤损伤。
