IgA肾病患者中IgA循环免疫复合物（CIC）的分子量：高效液相色谱法和固相抗C3 Facb酶免疫测定法的应用
Molecular weights of IgA-circulating immune complexes (CIC) in patients with IgA nephropathy: application of HPLC and solid-phase anti-C3 Facb EIA.
作者信息
Eguchi K, Tomino Y, Yagame M, Nomoto Y, Sakai H, Suguki H, Hosoda K
机构信息
Department of Internal Medicine, School of Medicine, Tokai University, Kanagawa, Japan.
出版信息
Tokai J Exp Clin Med. 1988 Feb;13(1):9-13.
The detection of molecular weights of IgA-circulating immune complexes (CIC) in sera is described. Measurement of IgA-, IgG- and IgM-CIC in sera was also performed. Serum samples were obtained from nine patients with IgA nephropathy, nine patients with chronic proliferative glomerulonephritis without IgA deposits (PGN) and 19 healthy adults. Serum samples of these patients were subjected to high performance liquid chromatography (HPLC) and then divided into 50 fractions. Measurement of IgA-, IgG- and IgM-CIC in such fractions was performed using solid-phase anti-C3 Facb EIA. The levels of IgA-CIC in sera from patients with IgA nephropathy were significantly higher than those from patients with PGN or healthy adults. The peak values of molecular weights of IgA-CIC in sera from patients with IgA nephropathy ranged from 2.6 X 10(5) to 3.0 X 10(5) daltons (mean +/- SD; 2.9 +/- 2.0). It was suggested that the increase of IgA-CIC in sera from patients with IgA nephropathy may be mainly due to dimers and/or polymers.
本文描述了血清中IgA循环免疫复合物（CIC）分子量的检测方法。同时还对血清中的IgA、IgG和IgM - CIC进行了测定。从9例IgA肾病患者、9例无IgA沉积的慢性增殖性肾小球肾炎（PGN）患者以及19名健康成年人中获取血清样本。这些患者的血清样本经过高效液相色谱（HPLC）分析，然后分为50个组分。使用固相抗C3 Facb酶免疫分析（EIA）对这些组分中的IgA、IgG和IgM - CIC进行测定。IgA肾病患者血清中IgA - CIC的水平显著高于PGN患者或健康成年人。IgA肾病患者血清中IgA - CIC分子量的峰值范围为2.6×10⁵至3.0×10⁵道尔顿（平均值±标准差；2.9±2.0）。提示IgA肾病患者血清中IgA - CIC的增加可能主要归因于二聚体和/或多聚体。
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