Yagame M, Tomino Y, Miura M, Tanigaki T, Suga T, Nomoto Y, Sakai H
Clin Exp Immunol. 1987 Feb;67(2):270-6.
The detection of circulating immune complexes (CIC) in sera from patients with IgA nephropathy is described. A solid-phase anti-C3 Facb enzyme immunoassay (EIA) was employed for detection of IgA-, IgG- and IgM-CIC in sera. The C1q-binding enzyme assay was also used for the detection of CIC in sera from these patients and healthy adults. Twenty-two patients with IgA nephropathy, 14 patients with other glomerular diseases and 19 healthy adults were examined by anti-C3 Facb EIA. The levels of IgA-CIC in sera from patients with IgA nephropathy were significantly higher than those in sera from patients with other glomerular diseases and healthy adults. CIC measured by the C1q-binding enzyme assay was detected in some patients with IgA nephropathy. The levels of serum IgA in patients with IgA nephropathy were significantly higher than those in patients with other glomerular diseases and healthy adults. However, there was no significant correlation between the levels of IgA-CIC in sera and those of serum IgA in patients with IgA nephropathy. There was also no significant correlation between the levels of IgA-CIC in sera and the degree of histopathological injuries in the patients. It is concluded that the solid-phase anti-C3 Facb EIA is useful for the detection of IgA-CIC in sera from patients with IgA nephropathy.
本文描述了对IgA肾病患者血清中循环免疫复合物（CIC）的检测。采用固相抗C3 Facb酶免疫测定法（EIA）检测血清中的IgA、IgG和IgM - CIC。C1q结合酶测定法也用于检测这些患者和健康成年人血清中的CIC。通过抗C3 Facb EIA对22例IgA肾病患者、14例其他肾小球疾病患者和19名健康成年人进行了检测。IgA肾病患者血清中IgA - CIC水平显著高于其他肾小球疾病患者和健康成年人血清中的水平。在一些IgA肾病患者中检测到通过C1q结合酶测定法测得的CIC。IgA肾病患者的血清IgA水平显著高于其他肾小球疾病患者和健康成年人。然而，IgA肾病患者血清中IgA - CIC水平与血清IgA水平之间无显著相关性。血清中IgA - CIC水平与患者组织病理学损伤程度之间也无显著相关性。结论是固相抗C3 Facb EIA可用于检测IgA肾病患者血清中的IgA - CIC。
