Chekmareva Natalia Y, Umriukhin Alexey E, Landgraf Rainer, Sotnikov Sergey V
Department of Behavioral Neuroendocrinology, Max Planck Institute of Psychiatry.
Department of Normal Physiology, Sechenov First Moscow State Medical University.
Behav Neurosci. 2019 Feb;133(1):68-76. doi: 10.1037/bne0000294.
This study focused on genetically determined versus acquired factors in shaping anxiety-related behavior by combining cross-breeding and cross-fostering approaches. Via cross-breeding of HAB (high anxiety-related behavior) female and LAB (low anxiety-related behavior) male mice, we obtained F1 hybrids with intermediate anxiety levels carrying genetic characteristics of both parental lines. Pups were raised either by their biological HAB (noncross-fostered control) or foster LAB (cross-fostered) mothers. Compared to controls, 6-week-old offspring raised by LAB mothers showed lower levels of anxiety in the elevated plus-maze and open field, but not the light-dark box, tests. No differences were found in the forced swim test reflecting active versus passive coping. The behavioral changes were associated with increased stress-induced concentrations of plasma corticosterone in cross-fostered animals. The expression of the corticotropin-releasing hormone receptor type I and glucocorticoid receptor genes did not differ in limbic and hypothalamic brain areas between cross-fostered and control mice. The data suggest that LAB-typical maternal care may partially shift behavioral and neuroendocrine characteristics of F1 crosses carrying both HAB and LAB alleles from intermediate toward reduced anxiety-related behavior. (PsycINFO Database Record (c) 2019 APA, all rights reserved).
本研究通过结合杂交和交叉寄养方法，聚焦于基因决定因素与后天获得因素在塑造焦虑相关行为中的作用。通过将高焦虑相关行为（HAB）的雌性小鼠与低焦虑相关行为（LAB）的雄性小鼠进行杂交，我们获得了具有中等焦虑水平的F1代杂种，它们携带了两个亲本品系的遗传特征。幼崽由亲生的HAB（非交叉寄养对照）或寄养的LAB（交叉寄养）母亲抚养。与对照组相比，由LAB母亲抚养的6周龄后代在高架十字迷宫和旷场试验中表现出较低的焦虑水平，但在明暗箱试验中没有差异。在反映主动应对与被动应对的强迫游泳试验中未发现差异。行为变化与交叉寄养动物中应激诱导的血浆皮质酮浓度增加有关。交叉寄养小鼠和对照小鼠在边缘脑区和下丘脑脑区中，促肾上腺皮质激素释放激素受体I型和糖皮质激素受体基因的表达没有差异。数据表明，LAB典型的母性照料可能会使携带HAB和LAB等位基因的F1代杂种的行为和神经内分泌特征部分地从中间水平转向降低焦虑相关行为。（PsycINFO数据库记录（c）2019美国心理学会，保留所有权利）
