Robert V, Verhave J P, Ponnudurai T, Louwé L, Scholtens P, Carnevale P
Antenne ORSTOM du Centre MURAZ, Bobo-Dioulasso, Burkina Faso.
Trans R Soc Trop Med Hyg. 1988;82(3):389-91. doi: 10.1016/0035-9203(88)90130-7.
Anopheles gambiae, experimentally infected with Plasmodium falciparum, were dissected 14 days later for microscopical detection of sporozoites and oocysts. The head, salivary glands, thorax, midgut, legs, ovaries, Malpighian tubules, the remainder of the abdominal tissues and the dissection fluid of each mosquito were examined by a two-site enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of circumsporozoite antigen (CS ag). 19 mosquitoes had CS ag in at least one of the specimens examined. Very large individual variability was observed in the presence and/or quantity of CS ag in the various parts. 7 mosquitoes were ELISA-positive in all 9 specimens; the salivary glands and thorax contained most CS ag, whereas the Malpighian tubules and ovaries contained the least; all the thoraces contained CS ag, even that of one mosquito of which the salivary glands lacked both sporozoites and CS ag; of 17 ELISA-positive salivary glands, 15 were found to contain sporozoites. The existence of free antigen associated with sporozoites, and the limitations of the ELISA technique in demonstrating the infectivity of a malaria vector, are discussed.
将感染了恶性疟原虫的冈比亚按蚊在14天后解剖，以显微镜检测子孢子和卵囊。通过双位点酶联免疫吸附测定（ELISA）对每只蚊子的头部、唾液腺、胸部、中肠、腿部、卵巢、马氏管、腹部其余组织和解剖液进行检测，以检测和定量环子孢子抗原（CS抗原）。在所检查的标本中，有19只蚊子至少在一个标本中检测到CS抗原。在各个部位CS抗原的存在和/或数量上观察到非常大的个体差异。7只蚊子在所有9个标本中ELISA检测呈阳性；唾液腺和胸部含有的CS抗原最多，而马氏管和卵巢含有的最少；所有胸部都含有CS抗原，甚至其中一只蚊子的唾液腺既没有子孢子也没有CS抗原；在17个ELISA检测呈阳性的唾液腺中，有15个发现含有子孢子。文中讨论了与子孢子相关的游离抗原的存在以及ELISA技术在证明疟疾媒介感染性方面的局限性。
