Beier J C, Perkins P V, Wirtz R A, Whitmire R E, Mugambi M, Hockmeyer W T
Am J Trop Med Hyg. 1987 May;36(3):459-68. doi: 10.4269/ajtmh.1987.36.459.
An enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody that recognizes a repetitive epitope on the circumsporozoite protein of Plasmodium falciparum was used in Kenya to assess malaria infections in Anopheles gambiae s.l. and An. funestus. The ELISA confirmed that 88% of 44 sporozoite-positive gland dissections were P. falciparum. The ELISA infection rate of 18.6% (n = 736) for individually tested mosquitoes for both species was significantly higher than the 10.4% (n = 537) salivary gland sporozoite rate determined by dissection. This difference was due to ELISA detection of medium and large sized oocysts on the midguts of infected mosquitoes which did not contain salivary gland sporozoites. From a series of 379 Anopheles that were cut at the thorax, ELISA tests on "head" and "body" portions showed that 29.5% of 95 positive mosquitoes contained circumsporozoite antigen in the body portion in the absence of salivary gland infections. This field evaluation demonstrates that the ELISA can most accurately be used to estimate sporozoite rates by cutting mosquitoes at the thorax and testing anterior portions.
在肯尼亚，使用一种单克隆抗体的酶联免疫吸附测定（ELISA）来评估冈比亚按蚊复合组和嗜人按蚊中的疟疾感染情况，该单克隆抗体可识别恶性疟原虫环子孢子蛋白上的重复表位。ELISA证实，在44个阳性子孢子腺体解剖样本中，88%为恶性疟原虫。两种蚊子个体检测的ELISA感染率为18.6%（n = 736），显著高于解剖确定的唾液腺子孢子率10.4%（n = 537）。这种差异是由于ELISA检测到感染蚊子中肠上存在中型和大型卵囊，但这些蚊子的唾液腺中没有子孢子。在一系列379只在胸部切开的按蚊中，对“头部”和“身体”部分进行的ELISA测试表明，95只阳性蚊子中有29.5%的蚊子身体部分含有环子孢子抗原，而唾液腺未感染。该现场评估表明，通过在胸部切开蚊子并检测前部，ELISA可最准确地用于估计子孢子率。
