Holguin Claudia M, Ma Xinyuan, Mueller John D, Agudelo Paula
Department of Agricultural and Environmental Sciences, Clemson University, Clemson, SC 29634.
Edisto Research and Education Center, Clemson University, Blackville, SC 29817.
Plant Dis. 2016 Jan;100(1):149-153. doi: 10.1094/PDIS-12-14-1332-RE. Epub 2015 Nov 12.
Hoplolaimus columbus is an important nematode pest of soybean in South Carolina and North Carolina. Tolerant cultivars are available for the management of this plant-parasitic nematode; however, variation in the response of soybean cultivars to H. columbus populations has been observed. This variation may be due to the presence of different species or high genetic diversity of H. columbus populations. The objective of this study was to identify the Hoplolaimus spp. present in fields representing the main soybean-growing regions in South Carolina and North Carolina and to examine the genetic variability of these populations. In South Carolina, the only species found associated with soybean was H. columbus but, in North Carolina, H. stephanus was the dominant species. The two species were never found together. Genetic variability analyses of a mitochondrial and a nuclear marker showed that only one haplotype was shared by the H. columbus populations. H. stephanus showed higher genetic variability, with private haplotypes per sampling location. Knowledge of the distribution and genetic variability of these two Hoplolaimus spp. is valuable to growers to determine potentially damaging infestations of these plant-parasitic nematodes in soybean fields.
哥伦比亚螺旋线虫是南卡罗来纳州和北卡罗来纳州大豆的一种重要线虫害虫。有耐性的品种可用于防治这种植物寄生线虫；然而，已观察到大豆品种对哥伦比亚螺旋线虫种群的反应存在差异。这种差异可能是由于哥伦比亚螺旋线虫种群中存在不同物种或高遗传多样性。本研究的目的是鉴定南卡罗来纳州和北卡罗来纳州主要大豆种植区田间存在的螺旋线虫种类，并研究这些种群的遗传变异性。在南卡罗来纳州，与大豆相关的唯一物种是哥伦比亚螺旋线虫，但在北卡罗来纳州，斯蒂芬螺旋线虫是优势物种。这两个物种从未同时被发现。对线粒体和核标记的遗传变异性分析表明，哥伦比亚螺旋线虫种群仅共享一个单倍型。斯蒂芬螺旋线虫显示出更高的遗传变异性，每个采样地点都有独特的单倍型。了解这两种螺旋线虫的分布和遗传变异性，对种植者确定大豆田这些植物寄生线虫潜在的破坏性侵染很有价值。
