膳食原儿茶酸通过抑制氧化损伤、炎症和 caspase-3 活性来纠正链脲佐菌素诱导的糖尿病大鼠的雄性生殖功能障碍。
Dietary protocatechuic acid abrogates male reproductive dysfunction in streptozotocin-induced diabetic rats via suppression of oxidative damage, inflammation and caspase-3 activity.
机构信息
Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Cancer Research and Molecular Biology Unit, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
出版信息
Eur J Pharmacol. 2019 Apr 15;849:30-42. doi: 10.1016/j.ejphar.2019.01.033. Epub 2019 Jan 26.
Clinical and experimental studies demonstrated that reproductive dysfunction is a non-lethal complication of diabetes. Protocatechuic acid (PCA) reportedly elicited several pharmacological effects in diabetic animals. However, there is paucity of information on the role of PCA in reproductive dysfunction associated with diabetes. The present study investigated the influence of PCA on the functional changes along the hypothalamic-pituitary-testicular axis in male diabetic rats. Streptozotocin (STZ)-induced diabetic rats were orally treated with PCA at 25 and 50 mg/kg body weight for 45 consecutive days. Results showed that PCA treatment significantly dwindled blood glucose level as well as prevented diabetes mediated decrease in body weight gain and organo-somatic indices of the testes and epididymis in the treated rats. Moreover, PCA increased the reproductive hormone levels, marker enzymes of testicular function and sperm functional characteristics in the treated rats. Further, PCA augmented the antioxidant status, inhibited lipid peroxidation and suppressed pro-inflammatory biomarkers including myeloperoxidase (MPO) activity, nitric oxide (NO) and tumor necrosis factor alpha (TNF-α) levels as well as caspase-3 activity inhypothalamus, testes and epididymis of diabetic rats. Collectively, PCA effectively abrogated reproductive deficits in diabetic rats via mechanisms involving suppression of oxidative stress, inflammation and caspase-3 activity along with enhancement of sperm functional parameters. Thus, PCA may preserve reproductive health in humans suffering from diabetes.
临床和实验研究表明，生殖功能障碍是糖尿病的一种非致命并发症。原儿茶酸（PCA）据称在糖尿病动物中具有多种药理作用。然而，关于 PCA 在与糖尿病相关的生殖功能障碍中的作用的信息很少。本研究调查了 PCA 对雄性糖尿病大鼠下丘脑-垂体-睾丸轴功能变化的影响。链脲佐菌素（STZ）诱导的糖尿病大鼠连续 45 天每天口服 PCA，剂量为 25 和 50mg/kg 体重。结果表明，PCA 治疗显著降低了血糖水平，并防止了糖尿病介导的体重增加和睾丸和附睾器官体重指数的下降。此外，PCA 增加了生殖激素水平、睾丸功能的标志物酶和精子功能特性。此外，PCA 增加了抗氧化状态，抑制了脂质过氧化，并抑制了包括髓过氧化物酶（MPO）活性、一氧化氮（NO）和肿瘤坏死因子-α（TNF-α）水平以及下丘脑、睾丸和附睾中 caspase-3 活性在内的促炎生物标志物糖尿病大鼠。总之，PCA 通过抑制氧化应激、炎症和 caspase-3 活性以及增强精子功能参数，有效地消除了糖尿病大鼠的生殖缺陷。因此，PCA 可能有助于保护患有糖尿病的人群的生殖健康。
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