Fang Manxin, Hu Wei, Liu Ben
College of Life Science and Resources and Environment, Yichun University, Yichun, China.
Engineering Technology Research Center of Jiangxi Universities and Colleges for Selenium Agriculture, Yichun University, Yichun, China.
Front Vet Sci. 2023 Sep 5;10:1228360. doi: 10.3389/fvets.2023.1228360. eCollection 2023.
Ochratoxin A (OTA) is a widely distributed mycotoxin. Nano-selenium (Nano-Se) is an emerging form of selenium known for its superior bioavailability, remarkable catalytic efficiency, and robust adsorbing capacity. Despite these characteristics, its impact on the microbial community and metabolomics in the cecum of chickens exposed to OTA has been infrequently investigated. This research examined the microbiota and metabolomic alterations linked to OTA in chickens, with or without Nano-Se present.
A cohort of 80 healthy chickens at the age of 1 day was randomly distributed into four groups of equal numbers, namely the Se cohort (1 mg/kg Nano-Se), the OTA cohort (50 μg/kg OTA), the OTA-Se cohort (50 μg/kg OTA + 1 mg/kg Nano-Se), and the control group. Each chicken group's caecal microbiome and metabolome were characterized using 16S rRNA sequencing and Liquid chromatography coupled with mass spectrometry (LC-MS) analyses.
Our results showed that the on day 21, the final body weight was significantly reduced in response to OTA treatments ( < 0.05), the average daily gain in the OTA group was found to be inferior to the other groups ( < 0.01). In addition, Nano-Se supplementation could reduce the jejunum and liver pathological injuries caused by OTA exposure. The 16S rRNA sequencing suggest that Nano-Se supplementation in OTA-exposed chickens mitigated gut microbiota imbalances by promoting beneficial microbiota and suppressing detrimental bacteria. Moreover, untargeted metabolomics revealed a significant difference in caecal metabolites by Nano-Se pretreatment. Collectively, the dataset outcomes highlighted that Nano-Se augmentation regulates intestinal microbiota and associated metabolite profiles, thus influencing critical metabolic pathways, and points to a possible food-additive product.
赭曲霉毒素A（OTA）是一种广泛分布的霉菌毒素。纳米硒（Nano-Se）是硒的一种新兴形式，以其卓越的生物利用度、显著的催化效率和强大的吸附能力而闻名。尽管具有这些特性，但纳米硒对暴露于OTA的鸡盲肠微生物群落和代谢组学的影响却鲜有研究。本研究调查了有无纳米硒存在情况下，鸡体内与OTA相关的微生物群和代谢组变化。
将80只1日龄健康鸡随机分为四组，每组数量相等，即硒组（1毫克/千克纳米硒）、OTA组（50微克/千克OTA）、OTA-硒组（50微克/千克OTA + 1毫克/千克纳米硒）和对照组。使用16S rRNA测序和液相色谱-质谱联用（LC-MS）分析对每组鸡的盲肠微生物组和代谢组进行表征。
我们的结果表明，在第21天，OTA处理导致最终体重显著降低（<0.05），OTA组的平均日增重低于其他组（<0.01）。此外，补充纳米硒可减轻OTA暴露引起的空肠和肝脏病理损伤。16S rRNA测序表明，在暴露于OTA的鸡中补充纳米硒可通过促进有益微生物群和抑制有害细菌来减轻肠道微生物群失衡。此外，非靶向代谢组学显示纳米硒预处理使盲肠代谢物有显著差异。总体而言，数据集结果突出表明，纳米硒增强可调节肠道微生物群和相关代谢物谱，从而影响关键代谢途径，并指向一种可能的食品添加剂产品。
