Department of Biotechnology, Khon Kaen University, Khon Kaen, 40002 Thailand.
Department of Biotechnology, Khon Kaen University, Khon Kaen, 40002 Thailand; Department of Food and Agricultural Product Technology, Gadjah Mada University, Yogyakarta, Indonesia.
J Biotechnol. 2019 Feb 20;292:57-63. doi: 10.1016/j.jbiotec.2019.01.003. Epub 2019 Jan 26.
The present study investigated cold hydrolysis of cassava pulp (CP) and the use of cold hydrolysis with simultaneous saccharification and fermentation (SSF) for ethanol production. Cold hydrolysis of 100 g-CP/L at 50 °C for 2 h, followed by at 30 °C for 72 h resulted in the production of 71.5 ± 1.8 g/L of reducing sugar, with a yield of 0.72 g/g-CP. A mathematical model describing the cold hydrolysis process was subsequently developed. The model proved to be applicable for other cold hydrolysis systems with satisfactory results. The sequential process of cold hydrolysis at 50 °C for 2 h, followed by SSF at 30 °C for 72 h gave 27.4 g-ethanol/L, with a productivity of 0.37 g/(L h) and a fermentation efficiency of 57.58%. Based on the results, a bioconversion process for CP to ethanol was proposed. In this process, 1 kg of ethanol could be produced from 3.65 kg of CP without any nutrient supplementation.
本研究探讨了木薯浆的低温水解以及低温水解与同步糖化发酵（SSF）在乙醇生产中的应用。在 50°C 下对 100g-CP/L 进行 2h 的低温水解，然后在 30°C 下进行 72h，可产生 71.5±1.8g/L 的还原糖，木薯浆得率为 0.72g/g-CP。随后开发了一个描述低温水解过程的数学模型。该模型被证明适用于其他低温水解系统，结果令人满意。在 50°C 下进行 2h 的低温水解，然后在 30°C 下进行 72h 的 SSF 顺序过程可得到 27.4g/L 的乙醇，产率为 0.37g/（L·h），发酵效率为 57.58%。基于这些结果，提出了一种将木薯浆转化为乙醇的生物转化工艺。在此过程中，无需任何营养补充，可从 3.65kg 的 CP 生产 1kg 的乙醇。
