Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Biosens Bioelectron. 2019 Mar 15;129:124-131. doi: 10.1016/j.bios.2019.01.029. Epub 2019 Jan 21.
A novel and sensitive sandwich-type photoelectrochemical (PEC) immunosensor was developed for the quantitative detection of β-amyloid protein (Aβ). A ITO electrode was sequentially coated with hierarchical porous zinc oxide (ZnO) microspheres with a large specific area, sensitized with tris(bipyridine)ruthenium(II) ion (Ru(bpy)) to achieve high visible light absorption, and modified with cerium-doped cadmium sulfide (Ce-CdS) nanoparticles to enhance the PEC response. Under the stimulation of visible light and ascorbic acid as an efficient electron donor, the photoelectric signal of ZnO/Ru(bpy)/Ce-CdS was 70 times that of pure ZnO. The amino-functionalized polystyrene (PS) microspheres coated with copper sulfide (CuS) was linked with a secondary antibody (Ab) for the first time for the Aβ detection by the immunosensor. The good insulation and steric resistance of the as-prepared polystyrene@CuS-Ab (PS@CuS-Ab) composite significantly weakened the photocurrent response of the immunosensor in the specific immune recognition. Under the optimal conditions, the quantitative detection of Aβ was achieved within the range of 0.001-100 ng/mL with the detection limit of 0.37 pg/mL. In addition, the PEC immunosensor is easy to make, stable and selective, which has provided a good experimental platform for the detection of disease biomarkers.
一种新颖且灵敏的夹心型光电化学（PEC）免疫传感器被开发出来，用于β-淀粉样蛋白（Aβ）的定量检测。ITO 电极依次涂覆具有大比表面积的分层多孔氧化锌（ZnO）微球，敏化剂为三（联吡啶）钌（II）离子（Ru（bpy））以实现高可见光吸收，并修饰铈掺杂硫化镉（Ce-CdS）纳米粒子以增强 PEC 响应。在可见光的刺激下，以及抗坏血酸作为高效电子供体，ZnO/Ru（bpy）/Ce-CdS 的光电信号是纯 ZnO 的 70 倍。首次将氨基功能化聚苯乙烯（PS）微球与硫化铜（CuS）结合，用于通过免疫传感器检测 Aβ。所制备的聚苯乙烯@硫化铜-抗体（PS@CuS-Ab）复合材料具有良好的绝缘性和空间位阻，在特异性免疫识别过程中显著减弱了免疫传感器的光电流响应。在最佳条件下，Aβ 的定量检测范围为 0.001-100ng/mL，检测限为 0.37pg/mL。此外，PEC 免疫传感器易于制造、稳定且具有选择性，为疾病生物标志物的检测提供了良好的实验平台。
