Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Mar 1;1109:19-24. doi: 10.1016/j.jchromb.2019.01.012. Epub 2019 Jan 22.
In this study, a high sensitive and reproducible method based on immunoaffinity cleanup followed by ultra-performance liquid chromatography-tandem mass spectrometry has been developed for the simultaneous determination bisphenol A (BPA) and bisphenol S (BPS) in aquatic products. The immunoaffinity column was prepared by coupling N-Hydroxysuccinimide-activated Sepharose 4B gel with monoclonal antibodies of BPA and BPS, which can selectively recognize the target analytes to achieve a low matrix interference. The matrix effects of different aquatic products were all less than ±10%. The limits of detection in four kinds of aquatic products was 0.060 μg/kg for BPA and 0.012 μg/kg for BPS. The recoveries of BPA and BPS in matrices varied from 74% to 106% with relative standard deviations <12%. The proposed method was successfully applied to real aquatic products.
本研究建立了一种基于免疫亲和净化结合超高效液相色谱-串联质谱法，用于同时检测水产品中双酚 A（BPA）和双酚 S（BPS）。免疫亲和柱是通过将 N-羟基琥珀酰亚胺活化的 Sepharose 4B 凝胶与 BPA 和 BPS 的单克隆抗体偶联制备而成，该抗体能够选择性地识别目标分析物，从而实现低基质干扰。不同水产品的基质效应均小于±10%。在四种水产品中的检测限分别为 BPA 的 0.060μg/kg 和 BPS 的 0.012μg/kg。BPA 和 BPS 在基质中的回收率在 74%至 106%之间，相对标准偏差<12%。该方法已成功应用于实际水产品。
