Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 10081, China.
Beijing University of Agriculture, Beijing 102206, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Jul 15;1178:122613. doi: 10.1016/j.jchromb.2021.122613. Epub 2021 Mar 9.
Bisphenol compounds (BPs) are a group of environmental contaminants with endocrine-disrupting effects both for humans and animals. The present work developed a sensitive analytical method for the detection of multiple BPs in the animal feed based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with post-column ammonium hydroxide (NHOH) infusion. A modified QuEChERS method was incorporated into the extraction and purification processes. The limit of detection (LODs) and quantification (LOQs) for the target BPs were in the ranges of 0.02-0.75 μg kg and 0.04-0.95 μg kg, respectively. Average recoveries were ranged between 82.6% and 112%. The proposed method was successfully applied to determine the concentrations of BPs in 20 actual feed samples, and the preliminary profiles of BPs in products from local feed factories were obtained. Each sample was simultaneously contaminated with at least 2 to 4 BPs, and bisphenol A (BPA) was the dominant analog of BPs found in animal feed.
双酚化合物（BPs）是一组具有内分泌干扰作用的环境污染物，对人类和动物都有影响。本工作开发了一种基于超高效液相色谱-串联质谱（UPLC-MS/MS）与柱后氨（NHOH）注入的动物饲料中多种 BPs 的灵敏分析方法。改良的 QuEChERS 方法被纳入提取和净化过程。目标 BPs 的检测限（LODs）和定量限（LOQs）分别在 0.02-0.75 μg kg 和 0.04-0.95 μg kg 范围内。平均回收率在 82.6%至 112%之间。该方法成功应用于 20 个实际饲料样品中 BPs 浓度的测定，并获得了当地饲料厂产品中 BPs 的初步概况。每个样品至少同时污染了 2 到 4 种 BPs，双酚 A（BPA）是动物饲料中 BPs 的主要类似物。
