Wingren S, Hatschek T, Stål O, Boeryd B, Nordenskjöld B
Department of Pathology I, Linköping University Hospital, Sweden.
Acta Oncol. 1988;27(6b):793-7. doi: 10.3109/02841868809094359.
DNA index, S-phase and G2/M fractions were measured in breast carcinomas before and after paraffin-embedding. Unfixed tumors were analysed with flow cytometry, while cells prepared from fixed and paraffin-embedded tumors were analysed with both static cytometry and flow cytometry. Cells for static cytometry were prepared with pepsin and cells for flow cytometry were prepared with trypsin. In 44 of 51 paraffin-embedded tumors, analysed with flow cytometry, both DNA index and S-phase fraction could be estimated. There was a strong correlation between the three methods of obtaining DNA indices and S-phase fractions, but this was not true for G2/M fractions. Histograms from flow cytometry of archival material were corrected for background. We conclude that cells prepared from formalin-fixed and paraffin-embedded breast cancer tissue can yield reliable estimation of DNA index and S-phase fraction.
在石蜡包埋前后对乳腺癌的DNA指数、S期和G2/M期比例进行了测量。未固定的肿瘤通过流式细胞术进行分析，而从固定和石蜡包埋的肿瘤中制备的细胞则通过静态细胞术和流式细胞术进行分析。用于静态细胞术的细胞用胃蛋白酶制备，用于流式细胞术的细胞用胰蛋白酶制备。在51个经石蜡包埋并通过流式细胞术分析的肿瘤中，有44个肿瘤的DNA指数和S期比例均可估计。获取DNA指数和S期比例的三种方法之间存在很强的相关性，但G2/M期比例并非如此。对存档材料的流式细胞术直方图进行了背景校正。我们得出结论，从福尔马林固定和石蜡包埋的乳腺癌组织中制备的细胞能够可靠地估计DNA指数和S期比例。
