Stål O, Klintenberg C, Franzen G, Risberg B, Arvidsson S, Bjelkenkrantz K, Skoog L, Nordenskjöld B
Breast Cancer Res Treat. 1986;7(1):15-22. doi: 10.1007/BF01886731.
332 primary invasive breast carcinomas were analysed by static cytofluorometry and flow cytometry. The ploidy distributions were similar, and 54% of the tumors were judged DNA aneuploid by both methods. The coefficient of variation of the G0-G1 peaks ranged from 2.0 to 8% with both techniques, but the mean was somewhat lower with flow cytometry--4.1%, compared to 4.9% for the static measurements. The proportion of S-phase cells was possible to estimate from 80% of the flow histograms and 70% of the static histograms. S-phase was not estimated from the static histograms if less than 150 tumor cells were measured. With 160 tumors S-phase was measured by both methods. The range was 0 to 27% with the static measurements and 0.7 to 25% with flow cytometry. Corresponding mean values were 7.6% and 8.2%, which are similar to thymidine labeling index results with breast cancers reported in some studies. A close correlation was obtained (r = 0.927) comparing S-phase fractions estimated from aneuploid tumors with flow cytometry and static cytofluorometry if more than 200 cells were measured with the latter. The proportion of S-phase cells was significantly lower for the diploid tumors. We conclude that both techniques can be useful for the estimation of DNA ploidy and replication in human breast cancer.
对332例原发性浸润性乳腺癌进行了静态细胞荧光测定法和流式细胞术分析。倍性分布相似，两种方法均判断54%的肿瘤为DNA非整倍体。两种技术的G0-G1峰变异系数范围均为2.0%至8%，但流式细胞术的平均值略低——为4.1%，而静态测量值为4.9%。80%的流式细胞直方图和70%的静态直方图能够估算S期细胞比例。如果测量的肿瘤细胞少于150个，则不根据静态直方图估算S期。对160例肿瘤采用两种方法测量S期。静态测量值范围为0至27%，流式细胞术测量值范围为0.7至25%。相应的平均值分别为7.6%和8.2%，与一些研究报道的乳腺癌胸苷标记指数结果相似。如果用静态细胞荧光测定法测量超过200个细胞，将流式细胞术和静态细胞荧光测定法估算的非整倍体肿瘤的S期分数进行比较，可得到密切相关性（r = 0.927）。二倍体肿瘤的S期细胞比例显著较低。我们得出结论，两种技术都可用于评估人类乳腺癌中的DNA倍性和复制情况。
