Universidad Autónoma de Nayarit, Secretaría de Investigación y Posgrado, Laboratorio de Inmunotoxicología, Boulevard Tepic-Xalisco s/n. Cd, de la Cultura Amado Nervo, C.P. 63000 Tepic, Nayarit, Mexico.
Centro Nayarita de Innovación y Transferencia de Tecnología A.C., Laboratorio Nacional para la Investigación en Inocuidad Alimentaria-Unidad Nayarit, Calle Tres s/n. Cd Industrial, Tepic, Nayarit, Mexico.
J Neuroimmunol. 2019 Mar 15;328:105-107. doi: 10.1016/j.jneuroim.2019.01.012. Epub 2019 Jan 22.
Nervous and immune systems maintain a bidirectional communication, expressing receptors for neurotransmitters and cytokines. Despite being well established in mammals, this has been poorly described in lower vertebrates as fishes. Experimental evidence shows that the neurotransmitter acetylcholine (ACh) regulates the immune response. In this research, we evaluated mRNA levels of muscarinic acetylcholine receptor (mAChR) in spleen mononuclear cells of Nile tilapia (Oreochromis niloticus) and compared the expression levels of immune cells with the brain. The mAChR subtypes (M2-M5A) were detected in both tissues, but mAChRs mRNA levels were higher in immune cells. This data have a potential use in biomedical and comparative immunology fields.
神经系统和免疫系统保持着双向交流，表达神经递质和细胞因子的受体。尽管在哺乳动物中已经得到很好的描述，但在鱼类等低等脊椎动物中描述得很少。实验证据表明，神经递质乙酰胆碱 (ACh) 可以调节免疫反应。在这项研究中，我们评估了尼罗罗非鱼（Oreochromis niloticus）脾单核细胞中毒蕈碱乙酰胆碱受体 (mAChR) 的 mRNA 水平，并比较了与大脑中免疫细胞的表达水平。在这两种组织中都检测到了 mAChR 亚型（M2-M5A），但免疫细胞中的 mAChR mRNA 水平更高。这些数据在生物医学和比较免疫学领域具有潜在的应用价值。
