一种对金鱼视觉系统具有特异性的单克隆抗体。

A monoclonal antibody specific for the visual system of the goldfish.

作者信息

Sharma S C, Braverman S B, Rappaport I

机构信息

Department of Ophthalmology, New York Medical College, Valhalla 10595.

出版信息

Brain Res. 1988 Nov 15;473(2):359-64. doi: 10.1016/0006-8993(88)90866-9.

DOI:10.1016/0006-8993(88)90866-9

PMID:3069185

Abstract

Although several monoclonal antibodies (MAbs) have been reported that recognize antigens present in ganglion cells of the mammalian retina, these MAbs do not cross-react in the goldfish. In the current study we have identified a MAb that recognized a 14.4 kDa antigen that is present on the ganglion cells in the retinae of goldfish but is absent from the retinae of all other species that were tested. No other cell type in the goldfish retina or optic nerve was labeled with this MAb. Furthermore, the axons in the optic nerve, optic tract and the terminal layers in the optic tectum were labeled.

摘要

尽管已经报道了几种识别哺乳动物视网膜神经节细胞中存在的抗原的单克隆抗体（MAb），但这些MAb在金鱼中不会发生交叉反应。在当前的研究中，我们鉴定出一种单克隆抗体，它识别一种14.4 kDa的抗原，该抗原存在于金鱼视网膜的神经节细胞上，但在所有其他测试物种的视网膜中均不存在。该单克隆抗体未标记金鱼视网膜或视神经中的其他细胞类型。此外，视神经、视束和视顶盖终末层中的轴突被标记。