Tumosa N, Stell W K
J Comp Neurol. 1986 Feb 8;244(2):267-75. doi: 10.1002/cne.902440212.
Published evidence that ganglion cells in the retinae of nonmammalian species are cholinergic is strong but indirect. In this paper we report results of attempts to demonstrate choline acetyltransferase immunoreactivity in ganglion cells of goldfish retina using two different antisera against choline acetyltransferase (ChAT), the acetylcholine-synthesizing enzyme. We obtained ChAT-immunoreactive staining of amacrine and displaced amacrine cells in the retina and type XIV cells in the tectum, but we obtained no direct immunocytochemical evidence that ganglion cells in the goldfish retina are cholinergic. Thus, ganglion cells identified by retrograde transport of propidium iodide were never ChAT-immunoreactive. Intraocular injections of colchicine did not result in the appearance of a population of ChAT-immunoreactive neurons in the ganglion cell layer. ChAT-immunoreactive axons were not observed in intact, ligated, or transected optic nerves. And finally, the ChAT immunoreactivity of cells and fibers in the optic tectum was unaffected by deafferentation. These experiments provide no positive evidence that any ganglion cells in goldfish retina contain the acetylcholine-synthesizing enzyme, ChAT. While it is possible that our method is too insensitive to detect the enzyme in ganglion cell somata or too specific to recognize the form of ChAT present in these cells, the fact that we can stain putatively cholinergic retinal amacrine cells and tectal neurons makes these alternative explanations improbable. We conclude that it is unlikely that any of the ganglion cells in the retina are cholinergic and that alternative explanations should be sought for previously published results that suggest that they are.
已发表的证据表明，非哺乳动物视网膜中的神经节细胞是胆碱能的，这一证据很有力但具有间接性。在本文中，我们报告了使用两种针对胆碱乙酰转移酶（ChAT，乙酰胆碱合成酶）的不同抗血清，试图在金鱼视网膜神经节细胞中证明胆碱乙酰转移酶免疫反应性的实验结果。我们在视网膜中获得了无长突细胞和移位无长突细胞以及顶盖中 XIV 型细胞的 ChAT 免疫反应性染色，但没有获得直接的免疫细胞化学证据表明金鱼视网膜中的神经节细胞是胆碱能的。因此，通过碘化丙啶逆行运输鉴定的神经节细胞从未有 ChAT 免疫反应性。眼内注射秋水仙碱并未导致神经节细胞层中出现一群 ChAT 免疫反应性神经元。在完整、结扎或横断的视神经中未观察到 ChAT 免疫反应性轴突。最后，顶盖中细胞和纤维的 ChAT 免疫反应性不受去传入神经支配的影响。这些实验没有提供积极的证据表明金鱼视网膜中的任何神经节细胞含有乙酰胆碱合成酶 ChAT。虽然有可能我们的方法对检测神经节细胞胞体中的酶过于不敏感，或者对识别这些细胞中存在的 ChAT 形式过于特异，但我们能够对假定为胆碱能的视网膜无长突细胞和顶盖神经元进行染色这一事实使得这些替代解释不太可能成立。我们得出结论，视网膜中的任何神经节细胞都不太可能是胆碱能的，对于先前发表的表明它们是胆碱能的结果，应该寻求其他解释。
