Leibniz Institute for Agricultural Engineering and Bioeconomy e.V. (ATB), Max-Eyth-Allee 100, 14469 Potsdam, Germany.
Leibniz Institute for Agricultural Engineering and Bioeconomy e.V. (ATB), Max-Eyth-Allee 100, 14469 Potsdam, Germany; Institute of Animal Hygiene and Environmental Health, Department of Veterinary Medicine, Free University Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany.
Waste Manag. 2019 Feb 1;84:74-82. doi: 10.1016/j.wasman.2018.11.028. Epub 2018 Nov 23.
The high prevalence of extended-spectrum-ß-lactamase (ESBL)-/AmpC-producing Escherichia (E.) coli in European broiler farms leads to the possible dissemination of antibiotic-resistant strains into the environment using contaminated feces as organic fertilizer. The aim of the present study was to determine the influence of temperature on the reduction kinetics of two artificially added ESBL-/AmpC-producing E. coli strains during lab-scale mesophilic (37 °C, 42 °C) and thermophilic (55 °C) anaerobic digestion of chicken manure. The decimal reduction times (D-value) were approximately 3-6 days at 37 °C, 1.5 days at 42 °C and 48 min at 55 °C. Starting with initial E. coli counts of 7 log colony forming units (CFU) per milliliter, both E. coli strains were below the detection limit after 35 days at all temperatures; however, at 37 °C and 42 °C, ESBL-producing E. coli were still partially detectable by enrichment. Temperature and retention time were the main inactivation factors. No direct correlation could be found between pH, volatile fatty acids (VFA) or ammonia (NH) and E. coli reduction. D-values were predicted for several temperatures between 37 °C and 55 °C and may help define time-temperature guidelines. Thermophilic digestion is an adequate method to rapidly inactivate ESBL-producing E. coli in chicken manure. At mesophilic temperatures, however, strict compliance of retention times and the prevention of short-circuiting become essential to gain an ESBL-producing E. coli free digestate.
欧洲肉鸡养殖场中广泛存在产超广谱β-内酰胺酶（ESBL）/AmpC 的大肠杆菌，这导致了携带抗生素抗性的菌株可能通过受污染的粪便作为有机肥料传播到环境中。本研究旨在确定温度对鸡粪在实验室规模的中温（37°C、42°C）和高温（55°C）厌氧消化过程中两种人工添加的产 ESBL/AmpC 大肠杆菌菌株的减少动力学的影响。在 37°C 时，D 值约为 3-6 天，在 42°C 时为 1.5 天，在 55°C 时为 48 分钟。从初始大肠杆菌计数为每毫升 7 个对数菌落形成单位（CFU）开始，在所有温度下，两种大肠杆菌菌株在 35 天后均低于检测限；然而，在 37°C 和 42°C 时，通过富集仍然可以部分检测到产 ESBL 的大肠杆菌。温度和保留时间是主要的失活因素。未发现 pH 值、挥发性脂肪酸（VFA）或氨（NH）与大肠杆菌减少之间存在直接相关性。在 37°C 和 55°C 之间的几个温度下预测了 D 值，这可能有助于确定时间-温度指南。高温消化是快速灭活鸡粪中产 ESBL 大肠杆菌的有效方法。然而，在中温条件下，严格遵守保留时间和防止短路成为获得无产 ESBL 大肠杆菌消化物的关键。
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