Chassot Francieli, Venturini Tarcieli Pozzebon, Piasentin Fernanda Baldissera, Santurio Janio Morais, Svidzinski Terezinha Inez Estivalet, Alves Sydney Hartz
Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, RS, Brazil.
Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, RS, Brazil.
Rev Iberoam Micol. 2019 Jan-Mar;36(1):44-47. doi: 10.1016/j.riam.2018.07.007. Epub 2019 Jan 26.
Candida parapsilosis may acquire resistance to echinocandins, a fact that prompts the search for new therapeutic options.
The present study aimed to evaluate the in vitro activity of antifungal agents, alone and in combination, against four groups of C. parapsilosis strains: (1) echinocandin-susceptible (ES) clinical isolates (MIC ≤ 2μg/ml), (2) anidulafungin-resistant strains (MIC ≥ 8μg/ml), (3) caspofungin-resistant strains (MIC ≥ 8μg/ml), and (4) micafungin-resistant strains (MIC ≥ 8μg/ml).
Antifungal interactions were evaluated by a checkerboard micro-dilution method. The determination of the MIC to each drug for every isolate according to the Clinical and Laboratory Standards Institute documents M27 (2017) and M60 (2017) was also done.
The echinocandins-resistant (ER) strains showed higher MICs to the tested antifungals than the ES strains, except for amphotericin B, for which the ER groups remained susceptible.
Most combinations showed indifferent interactions. The use of monotherapy still seems to be the best option. As resistance to echinocandins is an emergent phenomenon, further studies are required to provide clearer information on the susceptibility differences between strains to these antifungal agents.
近平滑念珠菌可能对棘白菌素产生耐药性，这一事实促使人们寻找新的治疗选择。
本研究旨在评估抗真菌药物单独及联合使用对四组近平滑念珠菌菌株的体外活性：（1）对棘白菌素敏感（ES）的临床分离株（MIC≤2μg/ml），（2）对阿尼芬净耐药的菌株（MIC≥8μg/ml），（3）对卡泊芬净耐药的菌株（MIC≥8μg/ml），以及（4）对米卡芬净耐药的菌株（MIC≥8μg/ml）。
采用棋盘微量稀释法评估抗真菌药物的相互作用。还根据临床和实验室标准协会文件M27（2017年）和M60（2017年）对每个分离株测定每种药物的MIC。
除两性霉素B外，对棘白菌素耐药（ER）的菌株对所测试的抗真菌药物的MIC高于ES菌株，ER组对两性霉素B仍敏感。
大多数联合用药表现为无关相互作用。单药治疗似乎仍是最佳选择。由于对棘白菌素的耐药性是一种新出现的现象，需要进一步研究以提供关于这些抗真菌药物菌株间药敏差异的更清晰信息。
