Schnitzer J
Max-Planck-Institut für Hirnforschung, Abteilung Neuroanatomie, Frankfurt am Main, F.R.G.
Brain Res Dev Brain Res. 1988 Nov 1;44(1):59-72. doi: 10.1016/0165-3806(88)90118-6.
Glial markers, namely antibodies to glial fibrillary acidic protein (GFAP), vimentin, galactocerebroside (GC), 04 antigen, and 08 antigen, were used to study the development of neuroglial cells in the postnatal rabbit retina. In histological sections radially oriented Müller cells were detectable at birth. They were weakly vimentin-positive and their labeling intensity increased during further development. Few vimentin-labeled astrocytes, situated in the nerve fiber layer, were detectable at birth, but of these few many were also GFAP-positive. The number of GFAP-positive astrocytes, all of which co-labeled with vimentin antibodies, increased during the following days. From postnatal day (P) 9-10 onward, the vimentin labeling seen in GFAP-positive astrocytes began to decline. In the adult rabbit most GFAP-positive astrocytes were only weakly vimentin-stained; some astrocytes even lacked detectable amounts of vimentin. Thus, astrocytes were found to be strongly vimentin-positive at birth, and strongly GFAP-positive but weakly vimentin-labeled by adulthood. Such a transition was not observed for Müller cells, which lacked detectable amounts of GFAP at all postnatal stages studied. In single cell suspensions, cells positive for the cell surface markers 04 antigen and GC were first detected at P6-7, 08 antigen-labeled cells were found one day later. Thus, the third class of neuroglial cells in the rabbit retina, the oligodendrocytes, seems to develop about one week later than astrocytes and Müller cells.
胶质细胞标志物，即针对胶质纤维酸性蛋白（GFAP）、波形蛋白、半乳糖脑苷脂（GC）、O4抗原和O8抗原的抗体，被用于研究出生后兔视网膜中神经胶质细胞的发育。在组织学切片中，出生时可检测到呈放射状排列的Müller细胞。它们波形蛋白弱阳性，在进一步发育过程中其标记强度增加。出生时在神经纤维层中可检测到少量波形蛋白标记的星形胶质细胞，但其中许多也呈GFAP阳性。随后几天，所有与波形蛋白抗体共标记的GFAP阳性星形胶质细胞数量增加。从出生后第（P）9 - 10天起，在GFAP阳性星形胶质细胞中观察到的波形蛋白标记开始下降。在成年兔中，大多数GFAP阳性星形胶质细胞仅波形蛋白染色弱阳性；一些星形胶质细胞甚至检测不到波形蛋白。因此，发现星形胶质细胞在出生时波形蛋白强阳性，到成年时GFAP强阳性但波形蛋白标记弱阳性。对于Müller细胞未观察到这种转变，在所研究的所有出生后阶段，Müller细胞均检测不到GFAP。在单细胞悬液中，首先在P6 - 7检测到细胞表面标志物O4抗原和GC阳性的细胞，一天后发现O8抗原标记的细胞。因此，兔视网膜中的第三类神经胶质细胞，即少突胶质细胞，似乎比星形胶质细胞和Müller细胞发育晚约一周。
