Zhao Zhen-Hua, Chen Zhi-Ting, Zhou Rui-Ling, Zhang Xu, Ye Qin-Yong, Wang Yin-Zhou
Department of Neurology, Fujian Provincial Hospital, Provincial Clinical College of Fujian Medical University, Fuzhou, China.
Department of Neurology, Fujian Medical University Union Hospital, Fuzhou, China.
Front Aging Neurosci. 2019 Jan 14;10:438. doi: 10.3389/fnagi.2018.00438. eCollection 2018.
The diagnosis of PD might be in difficulty, especially in the early stages. Therefore, the identification of novel biomarkers is imperative for the diagnosis and monitoring disease progression in PD. DJ-1 and α-synuclein, are two proteins that are critically involved in the pathogenesis of PD, and they have been examined as disease biomarkers in studies. However, no study exists regarding DJ-1 in plasma neural-derived exosomes. In the present study, the levels of DJ-1 and α-synuclein in plasma neural-derived exosomes were studied together in order to investigate novel biomarkers for PD. DJ-1 and α-synuclein in plasma and plasma neural-derived exosomes of the patients with PD and controls were quantified by ELISAs. The data revealed that the levels of DJ-1 and α-synuclein in plasma neural-derived exosomes and the ratio of plasma neural-derived exosomal DJ-1 to total DJ-1 were significantly higher in patients with PD, compared with controls, while levels of the two proteins in plasma exhibited no difference between the patients with PD and controls. However, no relationship was identified between biomarkers and disease progression. In addition, significant positive correlations between DJ-1 and α-synuclein in plasma neural-derived exosomes were found in the patients with PD and in healthy individuals. We hypothesize that DJ-1 in plasma neural-derived exosomes may be used as a potential biomarker as α-synuclein in PD and they might participate in the mechanism of PD together.
帕金森病（PD）的诊断可能存在困难，尤其是在疾病早期。因此，识别新的生物标志物对于PD的诊断和监测疾病进展至关重要。DJ-1和α-突触核蛋白是两种与PD发病机制密切相关的蛋白质，并且在研究中已将它们作为疾病生物标志物进行检测。然而，关于血浆神经源性外泌体中的DJ-1尚无相关研究。在本研究中，同时检测了血浆神经源性外泌体中DJ-1和α-突触核蛋白的水平，以探究PD的新型生物标志物。采用酶联免疫吸附测定法（ELISAs）对PD患者和对照组的血浆、血浆神经源性外泌体中的DJ-1和α-突触核蛋白进行定量分析。数据显示，与对照组相比，PD患者血浆神经源性外泌体中DJ-1和α-突触核蛋白的水平以及血浆神经源性外泌体DJ-1与总DJ-1的比值显著升高，而PD患者与对照组血浆中这两种蛋白的水平无差异。然而，未发现生物标志物与疾病进展之间存在关联。此外，在PD患者和健康个体中均发现血浆神经源性外泌体中的DJ-1与α-突触核蛋白之间存在显著正相关。我们推测，血浆神经源性外泌体中的DJ-1可能作为PD中α-突触核蛋白的潜在生物标志物，并且它们可能共同参与PD的发病机制。
