Tanei Tomonori, Pradipta Ambara R, Morimoto Koji, Fujii Motoko, Arata Mayumi, Ito Akihiro, Yoshida Minoru, Saigitbatalova Elena, Kurbangalieva Almira, Ikeda Jun-Ichiro, Morii Eiichi, Noguchi Shinzaburo, Tanaka Katsunori
Department of Breast and Endocrine Surgery Graduate School of Medicine Osaka University 2-2-E10 Yamadaoka Suita Osaka 565-0871 Japan.
Biofunctional Synthetic Chemistry Laboratory RIKEN Cluster for Pioneering Research 2-1 Hirosawa Wako Saitama 351-0198 Japan.
Adv Sci (Weinh). 2018 Nov 27;6(2):1801479. doi: 10.1002/advs.201801479. eCollection 2019 Jan 23.
Clean operating margins in breast cancer surgery are important for preventing recurrence. However, the current methods for determining margins such as intraoperative frozen section analysis or imprint cytology are not satisfactory since they are time-consuming and cause a burden on the patient and on hospitals with a limited accuracy. A "click-to-sense" probe is developed based on the detection of acrolein, which is a substance released by oxidatively stressed cancer cells and can be visualized under fluorescence microscopy. Using live breast tissues resected from breast cancer patients, it is demonstrated that this method can quickly, selectively, and sensitively differentiate cancer lesion from normal breast gland or benign proliferative lesions. Since acrolein is accumulated in all types of cancers, this method could be used to quickly assess the surgical margins in other types of cancer.
乳腺癌手术中的切缘阴性对于预防复发很重要。然而，目前用于确定切缘的方法，如术中冰冻切片分析或印片细胞学检查，并不令人满意，因为它们耗时且给患者和医院带来负担，准确性有限。基于对丙烯醛的检测开发了一种“点击传感”探针，丙烯醛是氧化应激癌细胞释放的一种物质，可在荧光显微镜下可视化。使用从乳腺癌患者切除的活体乳腺组织，证明该方法可以快速、选择性和灵敏地将癌病变与正常乳腺腺体或良性增生性病变区分开来。由于丙烯醛在所有类型的癌症中都会积累，该方法可用于快速评估其他类型癌症的手术切缘。
