一种观察红藻条斑紫菜向光性反应的简单方法。
A Simple Procedure to Observe Phototropic Responses in the Red Seaweed Pyropia yezoensis.
作者信息
Takahashi Megumu, Mikami Koji
机构信息
Faculty of Bio-Industry, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan.
Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
出版信息
Methods Mol Biol. 2019;1924:121-130. doi: 10.1007/978-1-4939-9015-3_9.
The marine red seaweed Pyropia yezoensis exhibits phototropic responses in gametophyte and conchosporangia phases, but not in sporophytes. These responses are easily monitored with a simple culturing box that has one side open to allow for unilateral light irradiation within an incubator. Confirmation of phototropic responses is achieved by changing the direction of unilateral light irradiation via rotation of the culture dishes clockwise 90°.
海洋红藻条斑紫菜在配子体和壳孢子囊阶段表现出向光性反应，但在孢子体阶段则没有。使用一个简单的培养箱就可以轻松监测这些反应，该培养箱一侧开口，以便在培养箱内进行单侧光照。通过将培养皿顺时针旋转90°来改变单侧光照的方向，从而确认向光性反应。
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