Department of Cell Biology, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, 215006, China.
Department of Biology, School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Mol Biol Rep. 2024 Jul 16;51(1):816. doi: 10.1007/s11033-024-09724-x.
Pyropia yezoensis a commercially important red seaweed species, is susceptible to various microorganisms infections, among which bacterial infections are the most prominent ones. Pyropia yezoensis is often affected by harmful bacterial communities under high temperatures that can lead to its degradation and economic losses. The current study aimed to explore Pyropia yezoensis-associated microbiota and further identify potential isolates, which can degrade Pyropia yezoensis under high-temperature conditions.
The 16S rRNA gene sequencing was used to identify the agarolytic bacterial species. The results showed that Chromohalobacter sp. strain AZ6, Pseudoalteromonas sp. strain AZ, Psychrobacter sp. strain AZ3, Vibrio sp. strain AZ, and Halomonas sp. strain AZ07 exhibited algicidal properties as these strains were more abundant at high temperature (25 °C). Among the five isolated strains, the potential isolate Halomonas sp. strain AZ07 showed high production of agarolytic enzymes, including lipase, protease, cellulase, and amylase. This study confirmed that the isolated strain could produce these four different enzymes. The strain Halomonas AZ07 was co-treated with Pyropia yezoensis cells under two different temperature environments, including 13 °C and 25 °C. The degradation of Pyropia yezoensis occurred at the optimum temperature of 25 °C and effectively degraded their cell wall, proteins, lipids, and carbohydrates.
The successful cultivation of Pyropia yezoensis in coastal farm environments is dependent on specific temperature and environmental factors, and lower temperatures have been observed to be particularly beneficial for the survival and growth of Pyropia yezoensis. The temperature below 13 °C was confirmed to be the best niche for the symbiotic relationship of microbiota associated with Pyropia yezoensis for its growth, development, and production.
条斑紫菜是一种具有商业价值的红藻，易受各种微生物感染，其中以细菌感染最为突出。条斑紫菜常受到高温下有害细菌群落的影响，这可能导致其降解和经济损失。本研究旨在探索条斑紫菜相关微生物群，并进一步鉴定潜在的分离株，这些分离株可以在高温条件下降解条斑紫菜。
使用 16S rRNA 基因测序鉴定琼脂酶细菌种。结果表明，Chromohalobacter sp. 菌株 AZ6、Pseudoalteromonas sp. 菌株 AZ、Psychrobacter sp. 菌株 AZ3、Vibrio sp. 菌株 AZ 和 Halomonas sp. 菌株 AZ07 在高温（25°C）下表现出杀藻特性，因为这些菌株的丰度更高。在分离的 5 株菌中，潜在分离株 Halomonas sp. 菌株 AZ07 表现出较高的琼脂酶产生能力，包括脂肪酶、蛋白酶、纤维素酶和淀粉酶。本研究证实，该分离株可以产生这四种不同的酶。在两种不同的温度环境（13°C 和 25°C）下，将菌株 Halomonas AZ07 与条斑紫菜细胞共同处理。在最适温度 25°C 下，条斑紫菜发生降解，其细胞壁、蛋白质、脂类和碳水化合物有效降解。
在沿海农场环境中成功培养条斑紫菜取决于特定的温度和环境因素，较低的温度被观察到特别有利于条斑紫菜的生存和生长。13°C 以下的温度被证实是与条斑紫菜相关的微生物共生的最佳小生境，有利于其生长、发育和产物生成。
