铁线蕨原丝体向光性和向极性中的细胞骨架观察

Cytoskeleton Observation in Phototropism and Polarotropism of Adiantum Protonema.

作者信息

Wada Masamitsu

机构信息

Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo, Japan.

出版信息

Methods Mol Biol. 2019;1924:191-198. doi: 10.1007/978-1-4939-9015-3_15.

DOI:10.1007/978-1-4939-9015-3_15

PMID:30694476

Abstract

The distribution patterns of the cytoskeleton, i.e., microtubules and actin filaments, in the apical part of protonemal cells are unique and differ from those of other apical growing cells, such as moss and liverwort protonemata, fungal hyphae, and angiosperm pollen tubes. A ring structure composed of microtubules and actin filaments exists at the basal part of the apical dome of protonemal cells. The structure may control the protonemal diameter and growth direction. Herein, the methods of staining of both microtubules and actin filaments are described.

摘要

在原丝体细胞顶端，细胞骨架即微管和肌动蛋白丝的分布模式独特，与其他顶端生长的细胞不同，如苔藓和地钱原丝体、真菌菌丝以及被子植物花粉管。在原丝体细胞顶端圆顶的基部存在一个由微管和肌动蛋白丝组成的环状结构。该结构可能控制原丝体的直径和生长方向。在此描述微管和肌动蛋白丝的染色方法。