Wada Masamitsu
Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo, Japan.
Methods Mol Biol. 2019;1924:27-33. doi: 10.1007/978-1-4939-9015-3_3.
Fern protonemal cells grow at their apices as long, undivided filamentous cells toward red (or weak white) light and change their growth direction if the light direction is changed (i.e., phototropism). When protonemata growing between an agar surface and cover glass are irradiated with polarized red light through the glass on the protonemal side, they start growing at the point where the direction of the vibration plane of polarized light and the transition moment of the photoreceptor, which is parallel to the plasma membrane of the cell's apical part, are equal (i.e., polarotropism). Herein, the methods on how to induce and observe this protonemal phototropism and polarotropism are described.
蕨类原丝体细胞在其顶端朝着红光（或弱白光）生长，形成长的、未分裂的丝状细胞，并且如果光的方向改变，它们的生长方向也会改变（即向光性）。当生长在琼脂表面和盖玻片之间的原丝体通过原丝体一侧的玻璃用偏振红光照射时，它们会在偏振光振动平面的方向与光感受器的跃迁矩（与细胞顶端部分的质膜平行）相等的点开始生长（即极向性）。在此，描述了诱导和观察这种原丝体向光性和极向性的方法。
