Scheithauer Lina, Steinert Michael
Institut für Mikrobiologie, Technische Universität Braunschweig, Braunschweig, Germany.
Methods Mol Biol. 2019;1921:323-331. doi: 10.1007/978-1-4939-9048-1_21.
Legionnaires' disease is a severe pneumonia caused by inhalation of Legionella pneumophila. Although powerful infection models ranging from monocellular host systems to mammals were developed, numerous intra- and extracellular interactions of L. pneumophila factors with human lung tissue structures remain unknown. Therefore, we developed and applied a novel infection model for Legionnaires' disease comprising living human lung tissue explants (HLTEs). This model allows analyzing Legionella infections at a unique level of complexity and narrows the gap between current infection models and postmortem histopathology analyses of infected patients. Here we describe the infection of tumor-free pulmonary tissue samples from patients undergoing lobe- or pneumectomy because of lung cancer. The method comprises bacterial cultivation, preparation of HLTEs, and infection of HLTEs. The infected tissue samples allow to characterize tissue damage, bacterial localization, dissemination and growth kinetics, and the host's molecular response.
军团病是一种因吸入嗜肺军团菌而引起的严重肺炎。尽管已经开发出了从单细胞宿主系统到哺乳动物的强大感染模型，但嗜肺军团菌因子与人类肺组织结构的众多细胞内和细胞外相互作用仍不为人知。因此，我们开发并应用了一种针对军团病的新型感染模型，该模型包含活的人肺组织外植体（HLTEs）。这个模型能够在独特的复杂程度层面分析军团菌感染情况，并缩小了当前感染模型与受感染患者尸检组织病理学分析之间的差距。在此，我们描述了对因肺癌接受肺叶切除术或全肺切除术患者的无肿瘤肺组织样本进行的感染。该方法包括细菌培养、HLTEs的制备以及HLTEs的感染。受感染的组织样本有助于表征组织损伤、细菌定位、扩散和生长动力学，以及宿主的分子反应。
