Lebenka A Iu
Genetika. 1988 Nov;24(11):1935-9.
To study the action of methylation on transformation efficiency, pA2 was methylated in vitro with M. Sau3A, M. AluI, M. MspI, M. HpaII, M. PvuII, M. BcnI, M. MvaI and transformed into Saccharomyces cerevisiae. The results suggest that efficiency of transformation depends on specificity of methylation, the nature of methylcytosine (m5C or m4C).
为研究甲基化对转化效率的作用，用Sau3A甲基化酶、AluI甲基化酶、MspI甲基化酶、HpaII甲基化酶、PvuII甲基化酶、BcnI甲基化酶、MvaI甲基化酶在体外对pA2进行甲基化，然后将其转化到酿酒酵母中。结果表明，转化效率取决于甲基化的特异性、甲基胞嘧啶（m5C或m4C）的性质。
