Shevchenko A A, Matseliukh B P
Genetika. 1988 Nov;24(11):1940-6.
The analysis of Streptomyces antibioticus recombinants isolated under non-selective conditions for stability of inheritance of parental properties demonstrated the existence of non-stable diploids and stable haploid recombinants. Also, genetic analysis of heteroclones isolated on selective medium after plating spores of regenerants was conducted. The results of analysis of haploid recombinant and heteroclones pointed to linkage of genetic loci and the possibility to use the technique of protoplast fusion for establishment of genetic map of S. antibioticus.
对在非选择性条件下分离得到的重组天蓝色链霉菌进行分析，以研究亲本特性遗传稳定性，结果表明存在不稳定二倍体和稳定单倍体重组体。此外，还对再生体孢子接种在选择性培养基上分离得到的异核体进行了遗传分析。单倍体重组体和异核体的分析结果表明了基因座之间的连锁关系，以及利用原生质体融合技术构建天蓝色链霉菌遗传图谱的可能性。
