Purnamasari Maria, Cawthray Gregory R, Barbetti Martin J, Erskine William, Croser Janine S
The University of Western Australia (UWA) Institute of Agriculture and School of Plant Biology, Faculty of Science, UWA, WA, 6009, Australia.
UWA Institute of Agriculture and Centre for Plant Genetics and Breeding, UWA.
Plant Dis. 2015 Nov;99(11):1544-1549. doi: 10.1094/PDIS-12-14-1297-RE. Epub 2015 Aug 20.
Camelina sativa (L.) Crantz. has been proposed as a novel source of oilseed resistance to Sclerotinia rot (SR; causal agent Sclerotinia sclerotiorum (Lib.) de Bary). To assess factors likely important in determining the level of resistance to this pathogen, 30 diverse C. sativa genotypes were evaluated using a cotyledon test under controlled environmental conditions. Confirmed cotyledon SR-resistant (CS370) and SR-susceptible (CS2305) genotypes were assessed for camalexin production across time following inoculation at the 1-month vegetative stage of growth. There were significant differences among C. sativa genotypes in response to inoculation with S. sclerotiorum in terms of percent cotyledon disease index (%CDI), with the mean %CDI ranging from 30.9 to 69.4% across germplasm and confirmation screening, respectively. Genotype CS370 consistently showed low %CDI indicating high level of resistance to S. sclerotiorum, whereas CS2305 showed the highest %CDI value. These findings highlight the potential to develop highly SR-resistant cultivars of C. sativa by selection. Furthermore, liquid chromatographic analysis of leaves for both SR-resistant and SR-susceptible genotypes demonstrated that camalexin was produced when inoculated with S. sclerotiorum. However, camalexin production was not linked with disease severity in either genotype, indicating that SR resistance in C. sativa is independent of the level of camalexin production.
亚麻荠（Camelina sativa (L.) Crantz.）已被提议作为一种新型的抗油菜菌核病（SR；病原菌为核盘菌（Sclerotinia sclerotiorum (Lib.) de Bary））的油籽来源。为了评估可能对决定该病原菌抗性水平至关重要的因素，在可控环境条件下使用子叶试验对30个不同的亚麻荠基因型进行了评估。在生长的1个月营养阶段接种后，对已确认的抗子叶SR（CS370）和感SR（CS2305）基因型随时间的camalexin产生情况进行了评估。亚麻荠基因型在对接种核盘菌的反应方面，就子叶病指数百分比（%CDI）而言存在显著差异，在种质和确认筛选中，平均%CDI分别为30.9%至69.4%。基因型CS370始终显示出低%CDI，表明对核盘菌具有高水平抗性，而CS2305显示出最高的%CDI值。这些发现突出了通过选择培育高抗SR亚麻荠品种的潜力。此外，对抗SR和感SR基因型的叶片进行液相色谱分析表明，接种核盘菌时会产生camalexin。然而，两种基因型中camalexin的产生均与病害严重程度无关，这表明亚麻荠中的SR抗性与camalexin的产生水平无关。
