Centre for Crop and Disease Management, Curtin University, Bentley, WA 6102, Australia.
Agriculture and Food, Commonwealth Scientific and Industrial Research Organization, Floreat, WA 6104, Australia.
Plant Dis. 2021 Sep;105(9):2314-2324. doi: 10.1094/PDIS-02-21-0367-RE. Epub 2021 Oct 14.
is an important fungal pathogen of chickpea ( L.), and it can cause yield losses up to 100%. The wild progenitors are much more diverse than domesticated chickpea, and this study describes how this relates to resistance. Initially, the pathogenicity of nine Australian isolates was examined on three lines to develop a robust phenotyping assay, and significant differences in isolate aggressiveness were identified with six isolates being classed as highly aggressive and three as moderately aggressive. We identified two isolates, CU8.20 and CU10.12, to be highly aggressive and moderately aggressive, respectively. A subsequent phenotyping assay was conducted using the two isolates to evaluate 86 wild accessions ( and ) and two varieties for resistance to . A subset of 12 genotypes was further evaluated, and subsequently, two wild accessions with consistently high levels of resistance to were examined using the initially characterized nine isolates. Wild accessions Karab_084 and Deste_063 demonstrated consistent partial resistance to . There were significant differences in responses to across wild collection sites. The Cermik, Karabahce, and Destek sites' responses to the aggressive isolate CU8.20 ranged from resistant to susceptible, highlighting an interaction between isolate genotype and chickpea collection site for sclerotinia stem rot resistance. This is the first evidence of partial stem resistance identified in wild germplasm, which can be adopted in chickpea breeding programs to enhance resistance in future chickpea varieties.
是鹰嘴豆（L.）的一种重要真菌病原体，可导致高达 100%的产量损失。野生祖先比驯化的鹰嘴豆更加多样化，本研究描述了这种多样性与抗性的关系。最初，在三个系上检查了 9 个澳大利亚分离物的致病性，以开发一种稳健的表型分析方法，发现分离物的侵袭性存在显著差异，其中 6 个分离物被归类为高度侵袭性，3 个为中度侵袭性。我们确定了两个分离物 CU8.20 和 CU10.12，分别为高度侵袭性和中度侵袭性。随后使用这两个分离物进行了后续的表型分析，以评估 86 个野生 （和 ）和两个 品种对 的抗性。进一步评估了 12 个基因型的子集，随后使用最初表征的 9 个分离物检查了对 具有一致高水平抗性的两个野生 。野生 品系 Karab_084 和 Deste_063 对 表现出一致的部分抗性。野生 收集点对 的反应存在显著差异。Cermik、Karabahce 和 Destek 地点对侵袭性分离物 CU8.20 的反应范围从抗到感，突出了分离物基因型和鹰嘴豆收集地点之间对茎溃疡病抗性的相互作用。这是首次在野生 种质中鉴定出部分茎部抗性的证据，可用于鹰嘴豆育种计划，以提高未来鹰嘴豆品种的 抗性。
