高浓度 CO 促进葡萄（Vitis vinifera L. cv. 'Pinot noir'）组培苗光合作用，通过蛋白组学和转录组学分析发现与 RbcS 和 Rca 调控有关。
Elevated CO concentration promotes photosynthesis of grape (Vitis vinifera L. cv. 'Pinot noir') plantlet in vitro by regulating RbcS and Rca revealed by proteomic and transcriptomic profiles.
机构信息
College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, People's Republic of China.
出版信息
BMC Plant Biol. 2019 Jan 29;19(1):42. doi: 10.1186/s12870-019-1644-y.
BACKGROUND
Plant photosynthesis can be improved by elevated CO concentration (eCO). In vitro growth under CO enriched environment can lead to greater biomass accumulation than the conventional in micropropagation. However, little is know about how eCO promotes transformation of grape plantlets in vitro from heterotrophic to autotrophic. In addition, how photosynthesis-related genes and their proteins are expressed under eCO and the mechanisms of how eCO regulates RbcS, Rca and their proteins have not been reported.
RESULTS
Grape (Vitis vinifera L. cv. 'Pinot Noir') plantlets in vitro were cultured with 2% sucrose designated as control (CK), with eCO (1000 μmol·mol) as C0, with both 2% sucrose and eCO as Cs. Here, transcriptomic and proteomic profiles associated with photosynthesis and growth in leaves of V. vinifera at different CO concentration were analyzed. A total of 1814 genes (465 up-regulated and 1349 down-regulated) and 172 proteins (80 up-regulated and 97 down-regulated) were significantly differentially expressed in eCO compared to CK. Photosynthesis-antenna, photosynthesis and metabolism pathways were enriched based on GO and KEGG. Simultaneously, 9, 6 and 48 proteins were involved in the three pathways, respectively. The leaf area, plantlet height, qP, ΦPSII and ETR increased under eCO, whereas Fv/Fm and NPQ decreased. Changes of these physiological indexes are related to the function of DEPs. After combined analysis of proteomic and transcriptomic, the results make clear that eCO have different effects on gene transcription and translation. RbcS was not correlated with its mRNA level, suggesting that the change in the amount of RbcS is regulated at their transcript levels by eCO. However, Rca was negatively correlated with its mRNA level, it is suggested that the change in the amount of its corresponding protein is regulated at their translation levels by eCO.
CONCLUSIONS
Transcriptomic, proteomic and physiological analysis were used to evaluate eCO2 effects on photosynthesis. The eCO triggered the RbcS and Rca up-regulated, thus promoting photosynthesis and then advancing transformation of grape plantlets from heterotrophic to autotrophic. This research will helpful to understand the influence of eCO on plant growth and promote reveal the mechanism of plant transformation from heterotrophic to autotrophic.
背景
提高 CO 浓度（eCO）可以提高植物光合作用。在富含 CO 的环境下进行体外生长会导致生物量积累比常规微繁殖更多。然而，对于 eCO 如何促进葡萄组培苗从异养到自养的转化知之甚少。此外，eCO 如何调节 RbcS、Rca 及其蛋白以及 eCO 调节 RbcS、Rca 及其蛋白的机制尚未报道。
结果
以 2%蔗糖为对照（CK），以 1000μmol·mol 为 eCO（C0），以 2%蔗糖和 eCO 为 Cs，培养葡萄（Vitis vinifera L. cv. 'Pinot Noir'）组培苗。在此，分析了不同 CO 浓度下葡萄叶片光合作用和生长相关的转录组和蛋白质组谱。与 CK 相比，eCO 中共有 1814 个基因（465 个上调和 1349 个下调）和 172 个蛋白（80 个上调和 97 个下调）表达差异显著。GO 和 KEGG 分析显示光合作用天线、光合作用和代谢途径富集。同时，三个途径分别涉及 9、6 和 48 个蛋白。eCO 下叶片面积、苗高、qP、ΦPSII 和 ETR 增加，而 Fv/Fm 和 NPQ 减少。这些生理指标的变化与 DEPs 的功能有关。通过蛋白质组学和转录组学的综合分析，结果表明 eCO 对基因转录和翻译有不同的影响。RbcS 与其 mRNA 水平不相关，表明 eCO 调节 RbcS 数量的变化是在转录水平上进行的。然而，Rca 与其 mRNA 水平呈负相关，表明其相应蛋白数量的变化是在翻译水平上受到 eCO 的调节。
结论
通过转录组学、蛋白质组学和生理学分析来评估 eCO2 对光合作用的影响。eCO 触发 RbcS 和 Rca 的上调，从而促进光合作用，进而促进葡萄组培苗从异养到自养的转化。本研究有助于了解 eCO 对植物生长的影响，促进植物从异养到自养的转化机制的揭示。
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