Voutev Roumen, Mann Richard S
Department of Biochemistry and Molecular Biophysics and Systems Biology, Columbia University, Zuckerman Mind Brain Behavior Institute, 3227 Broadway, Quad 9A, New York, NY 10027
G3 (Bethesda). 2019 Apr 9;9(4):983-986. doi: 10.1534/g3.119.0002.
Molecular biology techniques have a large impact on biomedical research and the availability of diverse tools to perform genome manipulations advances the ease of executing complicated genetic research. Here, we introduce in the fruit fly another such tool by harnessing the phage recombinase TP901-1 to perform site-directed recombination that leads to recombinase-mediated cassette exchange (RMCE). The TP901-1 system complements already existing recombination systems and enhances genome engineering in the fruit fly and other organisms.
分子生物学技术对生物医学研究有很大影响，各种用于基因组操作的工具的出现提高了进行复杂基因研究的便利性。在此，我们通过利用噬菌体重组酶TP901-1在果蝇中引入另一种此类工具，以进行位点特异性重组，从而实现重组酶介导的盒式交换（RMCE）。TP901-1系统补充了现有的重组系统，并增强了果蝇及其他生物体中的基因组工程。
