Voutev Roumen, Mann Richard S
Departments of Biochemistry and Molecular Biophysics and Systems Biology, Jerome L. Greene Science Center, Columbia University, New York, NY 10027
G3 (Bethesda). 2018 May 4;8(5):1399-1402. doi: 10.1534/g3.118.200051.
Effective genome engineering should lead to a desired locus change with minimal adverse impact to the genome itself. However, flanking loci with site-directed recombinase recognition sites, such as those of the phage ΦC31 integrase, allows for creation of platforms for cassette exchange and manipulation of genomic regions in an iterative manner, once specific loci have been targeted. Here we show that a genomic locus engineered with inverted minimal phage ΦC31 attP/attB sites can undergo efficient recombinase-mediated cassette exchange (RMCE) in the fruit fly .
有效的基因组工程应能实现所需的位点改变，同时对基因组本身的负面影响最小。然而，一旦特定位点被靶向，带有位点特异性重组酶识别位点（如噬菌体ΦC31整合酶的识别位点）的侧翼位点，能够以迭代方式创建用于盒式交换和基因组区域操作的平台。在这里，我们展示了用反向最小噬菌体ΦC31 attP/attB位点工程改造的基因组位点，可在果蝇中进行高效的重组酶介导的盒式交换（RMCE）。
