Wang Shuzhen, Li Zhiliang, Guo Xudong, Fang Yuanping, Xiang Jun, Jin Weibin
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization; Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains; College of Life Science, Huanggang Normal University, Huanggang, 438000, Hubei Province, P.R. China.
Breed Sci. 2018 Dec;68(5):536-544. doi: 10.1270/jsbbs.18092. Epub 2018 Nov 23.
possesses valuable horticultural and medicinal properties. However, the genetic studies have been hindered due to the lack of genetic markers. Based on RNA-seq, large-scale molecular markers were developed from four species endemic to Dabie Mountains (central China): (5.25 Gb; SSRs, 12,756, one/2.37 kb, 147 types; SNPs, 38,313; InDels, 3,174), (5.80 Gb; SSRs, 13,294, one/2.58 kb, 167 types; SNPs, 136,590; InDels, 6,258), (6.53 Gb; SSRs, 15,724, one/2.51 kb, 170 types; SNPs, 44,942; InDels, 4,126), and (4.35 Gb; SSRs, 10,214, one/2.49 kb, 110 types; SNPs, 77,829; InDels, 3,416). Di-nucleotide repeats were the main type (59.126%-64.314%), and AG/CT repeat (55.18%-61.22%) was the most. In particular, 89 species-specific types had been found. Furthermore, C:G→T:A mutation was the main SNP type (30.475%-34.99%). However, C:G→G:C mutation was the least type in , while T:A→G:C mutation was the least in the other three species. InDels with length of 3 nt was most in , but 1 nt InDels were the main type in the other three species. Twelve microsatellite markers developed from reveled high genetic diversity in the four populations, and heterozygote excess was observed. This research would benefit the genetic study, molecular marker-assisted selection, and breeding studies in species.
具有重要的园艺和药用价值。然而，由于缺乏遗传标记，其遗传研究受到了阻碍。基于RNA测序，从大别山（中国中部）特有的四个物种中开发了大规模分子标记：（5.25 Gb；简单序列重复，12756个，每2.37 kb一个，147种类型；单核苷酸多态性，38313个；插入缺失，3174个），（5.80 Gb；简单序列重复，13294个，每2.58 kb一个，167种类型；单核苷酸多态性，136590个；插入缺失，6258个），（6.53 Gb；简单序列重复，15724个，每2.51 kb一个，170种类型；单核苷酸多态性，44942个；插入缺失，4126个），以及（4.35 Gb；简单序列重复，10214个，每2.49 kb一个，110种类型；单核苷酸多态性，77829个；插入缺失，3416个）。二核苷酸重复是主要类型（59.126%-64.314%），AG/CT重复（55.18%-61.22%）最为常见。特别地，发现了89种物种特异性类型。此外，C:G→T:A突变是主要的单核苷酸多态性类型（30.475%-34.99%）。然而，C:G→G:C突变在[物种名称未明确]中最少，而T:A→G:C突变在其他三个物种中最少。长度为3 nt的插入缺失在[物种名称未明确]中最多，但1 nt插入缺失是其他三个物种中的主要类型。从[物种名称未明确]开发的12个微卫星标记在四个种群中显示出高遗传多样性，并观察到杂合子过剩。本研究将有利于[物种名称未明确]物种的遗传研究、分子标记辅助选择和育种研究。
