杜鹃花科微卫星位点的分离与特征分析
Isolation and characterization of microsatellite loci for (Ericaceae).
作者信息
Pan Tao, Pei Ya-Li, Zhao Kai, Liu Xin-Yue, Wang Chen-Cheng, Zhang Bao-Wei
机构信息
School of Life Sciences Anhui University Hefei Anhui Province People's Republic of China.
School of Life Sciences Anhui Normal University Wuhu Anhui Province People's Republic of China.
出版信息
Appl Plant Sci. 2019 Feb 21;7(2):e01222. doi: 10.1002/aps3.1222. eCollection 2019 Feb.
PREMISE OF THE STUDY
We developed microsatellite primers for (Ericaceae), a narrowly distributed species found in the Dabie Mountains, China, to study the genetic diversity, population structure, and evolutionary history of the species.
METHODS AND RESULTS
Two terminal sequencing modes of the Illumina HiSeq platform were used to mine simple sequence repeat markers from large-scale transcriptional groups. In this study, 24 microsatellite loci were screened. The number of alleles ranged from one to 20, and the levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and 0.000 to 0.918, respectively. Most of these primers were successfully amplified in eight congeneric species (, , , , , , , and ).
CONCLUSIONS
These newly developed microsatellite loci will be useful for studying the genetic diversity and population structure of and congeneric species.
研究前提
我们开发了用于[某种杜鹃花科植物]的微卫星引物，该物种分布狭窄，在中国大别山区被发现，以研究该物种的遗传多样性、种群结构和进化历史。
方法与结果
利用Illumina HiSeq平台的两种末端测序模式，从大规模转录组中挖掘简单序列重复标记。在本研究中，筛选出24个微卫星位点。等位基因数从1到20不等，观察到的杂合度和预期杂合度水平分别从0.000到1.000和0.000到0.918不等。这些引物中的大多数在8个同属物种（[物种名称1]、[物种名称2]、[物种名称3]、[物种名称4]、[物种名称5]、[物种名称6]、[物种名称7]和[物种名称8]）中成功扩增。
结论
这些新开发的微卫星位点将有助于研究[该物种名称]及其同属物种的遗传多样性和种群结构。
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