意大利熏衣草对副溶血性弧菌产毒及生长的影响

The Effect of Lavandula Stoechas on Toxigenesis and the Growth of Vibrio Parahaemolyticus.

作者信息

Maghsoudi Omid, Zeraatkar Mohsen, Dolatabadi Mojtaba, Johari Ahmad, Barati Karizno Mahdi, Ranjbar Reza

机构信息

Doctor of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Iran.

Dept. of Food Hygiene, Ferdowsi University of Mashhad, Mashhad, Iran.

出版信息

Iran J Pathol. 2018 Spring;13(2):245-255. Epub 2018 Jul 17.

PMID:30697296

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6339501/

Abstract

BACKGROUND & OBJECTIVE: Outbreak of food-borne diseases has become more and more important these days and using natural food preservers with high durability is under debate. Vegetative essence is a type of food preserver and many studies have been performed on their antimicrobial effects. The purpose of this study wasto investigate antibacterial effects of lavender essence on toxigenesis and the growth of .

METHODS

lavender essence was prepared and its components were identified using GCMS. Determining minimum interceptorgrowth of was assessed in test tubes containing BHI. Thermal resistant hemolysin was measured by Kap-RPLAkit. Growth diagram was prepared after determining toxin formation titration of the bacterium during 0, 2, 3, 4, 6, 8 and 24 hours.

RESULTS

Cineol, Borneol, Camphor, LinaloolL and Alpha-pinen had the highest concentrations in the essence, respectively. Results of minimum intercepter concentration of lavender (0, 0.005, 0.015, 0.03 and 0.045 percent) on showed that 0.03% and higher concentrations had the ability to prevent growth and toxin formation of . In addition, the effect of different concentrations of essence on toxin titration of bacterium showed no toxin at concentrations of 0.030 and 0.045.

CONCLUSION

lavender essence was able to prevent the growth and toxin formation of Vibrio parahaemolyticus.

摘要

背景与目的

如今食源性疾病的爆发变得越来越重要，使用具有高耐久性的天然食品防腐剂正处于讨论之中。植物精华是一种食品防腐剂，并且已经对其抗菌作用进行了许多研究。本研究的目的是调查薰衣草精华对产毒和[细菌名称未完整]生长的抗菌作用。

方法

制备薰衣草精华并使用气相色谱 - 质谱联用仪（GCMS）鉴定其成分。在含有脑心浸液（BHI）的试管中评估[细菌名称未完整]的最小抑菌浓度。通过卡普 - 赖特氏血浆凝固酶试验试剂盒（Kap - RPLA kit）测量耐热溶血素。在确定细菌在0、2、3、4、6、8和24小时期间的毒素形成滴定后绘制生长图。

结果

桉叶素、冰片、樟脑、芳樟醇和α - 蒎烯在精华中的浓度分别最高。薰衣草（0、0.005、0.015、0.03和0.045百分比）对[细菌名称未完整]的最小抑菌浓度结果表明，0.03%及更高浓度能够阻止[细菌名称未完整]的生长和毒素形成。此外，不同浓度精华对细菌毒素滴定的影响表明，在0.030和0.045浓度下没有毒素。