Garikapati Devala Rao, Shaik Praveen Begum, Penchalaiah Hanumanthu
K.V.S.R. Siddhartha College of Pharmaceutical Sciences Vijayawada, India.
Research Scholar, University College of Pharmaceutical Sciences, Acharya Nagarjuna University Nagarjuna Nagar, Guntur, India.
Int J Physiol Pathophysiol Pharmacol. 2018 Dec 25;10(6):184-191. eCollection 2018.
Chronic unpredictable stressors can produce a situation similar to clinical depression, and such animal models can be used for the preclinical evaluation of antidepressants. Many findings have shown that the levels of proinflammatory cytokines (e.g., TNF-α) and oxidative stress (increased lipid peroxidation, decreased glutathione levels, and endogenous antioxidant enzyme activities) are increased in patients with depression. Silibinin is the major active constituent of silymarin, a standardized extract of the milk thistle seeds, containing a mixture of flavonolignans consisting of silibinin, isosilibinin, silicristin, silidianin and exhibit antioxidant activity. Objectives The present study was designed to investigate the effect of silibinin on unpredictable chronic stressinduce behavioral and biochemical alterations in mice.
Mice were subjected to different stress paradigms daily for a period of 45 days to induce depressive like behavior such as memory acquisition, and retention.
Chronic treatment with silibinin significantly reversed the unpredictable chronic stress-induced behavioral (improve memory function), biochemical changes (decreased glutathione levels, superoxide dismutas), and inflammation surge (serum TNF-α IL 1β) in stressed mice.
The study revealed that silibinin exerted effects in behavioral despair paradigm in chronically stressed mice, specifically by modulating central oxidative stress and inflammation.
慢性不可预测应激源可产生类似于临床抑郁症的情况，此类动物模型可用于抗抑郁药的临床前评估。许多研究结果表明，抑郁症患者体内促炎细胞因子（如肿瘤坏死因子-α）水平和氧化应激（脂质过氧化增加、谷胱甘肽水平降低以及内源性抗氧化酶活性降低）升高。水飞蓟宾是水飞蓟素的主要活性成分，水飞蓟素是水飞蓟种子的标准化提取物，含有由水飞蓟宾、异水飞蓟宾、水飞蓟宁、水飞蓟亭组成的黄酮木脂素混合物，并具有抗氧化活性。目的本研究旨在探讨水飞蓟宾对不可预测慢性应激诱导的小鼠行为和生化改变的影响。
每天对小鼠进行不同的应激模式处理，持续45天，以诱导类似抑郁的行为，如记忆获取和保持。
水飞蓟宾的慢性治疗显著逆转了不可预测慢性应激诱导的应激小鼠的行为（改善记忆功能）、生化变化（谷胱甘肽水平降低、超氧化物歧化酶）和炎症激增（血清肿瘤坏死因子-α、白细胞介素1β）。
该研究表明，水飞蓟宾在慢性应激小鼠的行为绝望模型中发挥作用，特别是通过调节中枢氧化应激和炎症。
