Larsson E L, Blomgren H
Int Arch Allergy Appl Immunol. 1978;57(4):333-40. doi: 10.1159/000232122.
Cultures of lymphocytes stimulated by polyclonal mitogens or specific antigens exhibit an initial wave of cell proliferation followed by a decline which has been explained by the activation of suppressor T cells. This article presents the first results aiming at testing the possibility that the suppressor cells are activated by soluble products released by stimulated lymphocytes. It was found that the supernatants of PHA-activated human lymphocytes stimulate lymphocytes to DNA synthesis with peak responses occurring on day 5 independent of the cell concentration in the cultures. In contrast, the kinetics of lymphocyte responses to phytomitogens or in the mixed lymphocyte culture were found to be dependent on cell concentration; peak responses were delayed at decreasing cell concentrations. The results support the view that certain lymphokines released in stimulated lymphocyte cultures induce suppressor cells and that biologically active concentrations are reached earlier at high cell densities.
由多克隆有丝分裂原或特异性抗原刺激的淋巴细胞培养物表现出细胞增殖的初始波，随后是下降，这已通过抑制性T细胞的激活得到解释。本文展示了首个旨在测试抑制性细胞是否由受刺激淋巴细胞释放的可溶性产物激活的可能性的结果。研究发现，PHA激活的人淋巴细胞的上清液刺激淋巴细胞进行DNA合成，在第5天出现峰值反应，且与培养物中的细胞浓度无关。相比之下，发现淋巴细胞对植物有丝分裂原或混合淋巴细胞培养的反应动力学取决于细胞浓度；随着细胞浓度降低，峰值反应延迟。这些结果支持这样一种观点，即受刺激淋巴细胞培养物中释放的某些淋巴因子会诱导抑制性细胞，并且在高细胞密度下更早达到生物活性浓度。
