受刺激的人淋巴细胞释放的可溶性产物对淋巴细胞增殖的调节。

Regulation of lymphocyte proliferation by soluble products released by stimulated human lymphocytes.

作者信息

Blomgren H, Larsson E L

出版信息

Int Arch Allergy Appl Immunol. 1978;57(1):15-21. doi: 10.1159/000232078.

DOI:10.1159/000232078

Abstract

Human lymphocytes pulsed with phytothaemagglutinin (PHA) release mitogenic factors and other lymphokines upon subsequent incubation in fresh medium. Such active supernatants (SUPs) were assayed for their capacity to modify the DNA synthetic response of human lymphocytes exposed to various mitogenic stimuli. Both non-fractionated peripheral lymphocytes and preparations of fractionated T cells were stimulated by active SUPs to DNA synthesis. In contrast, active SUPs reduced the responses of lymphocytes to allogeneic cells, PPD tuberculin and PHA. These reductions were most evident at the end of the culture periods. The results suggest that soluble products released by stimulated lymphocytes activate suppressor lymphocytes which inhibit proliferative responses of lymphocytes.

摘要

用植物血凝素（PHA）刺激的人淋巴细胞在随后于新鲜培养基中孵育时会释放促有丝分裂因子和其他淋巴因子。对这种活性上清液（SUPs）进行检测，以评估其改变暴露于各种促有丝分裂刺激下的人淋巴细胞DNA合成反应的能力。未分级的外周淋巴细胞和分级T细胞制剂均受到活性SUPs刺激而发生DNA合成。相比之下，活性SUPs降低了淋巴细胞对同种异体细胞、结核菌素纯蛋白衍生物（PPD）和PHA的反应。这些降低在培养期结束时最为明显。结果表明，受刺激淋巴细胞释放的可溶性产物激活了抑制淋巴细胞增殖反应的抑制性淋巴细胞。