Barreto-Durán Emilia, Mejía-Cruz Claudia Camila, Leal-García Efrain, Pérez-Núñez Rafael, Rodríguez-Pardo Viviana Marcela
Immunobiology and Cell Biology Group, Department of Microbiology, Science Faculty, Pontificia Universidad Javeriana Bogotá D.C., Colombia, South America.
Department of Orthopedics and Traumatology, School of Medicine, Pontificia Universidad Javeriana, Hospital Universitario San Ignacio Bogotá D.C., Colombia, South America.
Am J Stem Cells. 2018 Dec 1;7(5):114-120. eCollection 2018.
In recent years, the therapeutic use of mesenchymal stromal cells (MSC) has generated a valuable number of scientific studies that delve into their biological characteristics and their potential in regenerative medicine; however, the impact of the clinical characteristics of tissue donors, from which these cells are isolated, on their potential in applied clinical research is not yet clear. The objective of this study was to evaluate the impact of the clinical characteristics of bone marrow donors on the quality of this tissue as a source of MSC for therapeutic use. Human MSC were isolated, characterized and cultured (according to ISCT criteria) from bone marrow samples from volunteer donors (n = 70) attending the Department of Orthopedics and Traumatology of the Hospital Universitario San Ignacio (Bogota, Colombia) for surgery of prosthetic hip replacement that agreed to participate voluntarily in the study. Donor data such as age, gender, weight, smoker and type of anesthesia used during the surgical procedure were recorded, and the impact of these characteristics on the volume of tissue collection, mononuclear cell count and confluence time of cells with fibroblastoid morphology was evaluated. Correlation coefficients between quantitative variables were calculated with Spearman's correlation test, and the association between qualitative and quantitative variables was evaluated with biserial correlation coefficient. A significant correlation was observed between the age of the donors and the time necessary to obtain confluent cells in vitro (r = 0.2489, P = 0.0377); similarly, the correlation between the volume of bone marrow collected and the number of mononuclear cells obtained was significant (r = 0.7101, P = 0.0001). Although a negative correlation tendency was observed between the mononuclear cell count and the confluence time, this was not significant (r = -0.2041, P = 0.0950). No significant associations were observed between gender, smoking status or type of anesthesia and the expansion characteristics of human mesenchymal stromal cells. Bone marrow donor age and the tissue collection volume impact the time of obtaining MSC in vitro and the mononuclear cell count with which the culture starts. These conditions must be considered when the bone marrow is selected as the tissue for obtaining MSC.
近年来，间充质基质细胞（MSC）的治疗应用催生了大量科学研究，这些研究深入探究了它们的生物学特性及其在再生医学中的潜力；然而，分离这些细胞的组织供体的临床特征对其在应用临床研究中的潜力的影响尚不清楚。本研究的目的是评估骨髓供体的临床特征对作为治疗用MSC来源的该组织质量的影响。从圣伊格纳西奥大学医院（哥伦比亚波哥大）骨科和创伤科接受人工髋关节置换手术且自愿同意参与本研究的志愿者供体（n = 70）的骨髓样本中分离、鉴定并培养（根据国际细胞治疗协会标准）人MSC。记录供体数据，如年龄、性别、体重、是否吸烟以及手术过程中使用的麻醉类型，并评估这些特征对组织采集量、单核细胞计数以及具有成纤维细胞样形态的细胞汇合时间的影响。使用Spearman相关检验计算定量变量之间的相关系数，并使用双列相关系数评估定性和定量变量之间的关联。观察到供体年龄与体外获得汇合细胞所需时间之间存在显著相关性（r = 0.2489，P = 0.0377）；同样，采集的骨髓量与获得的单核细胞数量之间的相关性也很显著（r = 0.7101，P = 0.0001）。尽管观察到单核细胞计数与汇合时间之间存在负相关趋势，但不显著（r = -0.2041，P = 0.0950）。未观察到性别、吸烟状况或麻醉类型与人间充质基质细胞的扩增特性之间存在显著关联。骨髓供体年龄和组织采集量会影响体外获得MSC的时间以及培养开始时的单核细胞计数。当选择骨髓作为获取MSC的组织时，必须考虑这些条件。
